FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3025741681> ?p ?o ?g. }

• W3025741681 endingPage "37" @default.
• W3025741681 startingPage "29" @default.
• W3025741681 abstract "Aim. The aim of study was to develop a simple and accurate procedure that could be applied for the determination of impurities and degradation products in cardiazol. Materials and methods. Separation in samples was carried out with Acquity H-class UPLC system (Waters, Milford, USA) equipped with Acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm) (Waters, Milford, USA). Xevo TQD triple quadrupole mass spectrometer detector (Waters Millford, USA) was used to obtain MS/MS data. Mobile phase A: 0.1% solution of trifluoroacetic acid R in water R ; Mobile phase B: acetonitrile R . Samples were chromatographed in gradient mode (Table 1). Flow rate of the mobile phase: 1 ml / min. Column temperature: 30 °С. Detection: at 240 nm wavelength. Injection volume: 10 μl. Results. The retention time of the main substance is about 18.5 minutes. The order of the peak, the retention times and relative retention times: impurity B (12.04, 0.65); impurity А (18.5; 0.98); Cardiazol (18.87; 1.00). The LOD and LOQ values obtained were in the range of 30 ng/mL to 100 ng/mL and 80 ng/mL to 310 ng/mL respectively (with respect to sample concentration of 2 mg/ml). Linearity was established in the range of LOQ level to 0.2% having regression coefficients in the range of 0.9996 to 0.9999. The change in the temperature of the column affects the degree of separation of cardiazol and the impurity A, and thus, with a decrease of 5 ° C, the degree of separation is (1.06), while with increasing this index (3.43). When changing the flow rate of the mobile phase, the degree of separation changes in the following order, with a decrease to 0.9 ml / min separation (1.90), with an increase in speed to 1.1 ml / min (2.45). When the number of mobile phase B decreases by 5%, the degree of separation varies by (2.65), with an increase of 5% (1.82). In comparison with the chromatogram of the tested solution, the substance is not resistant to the action of peroxide, alkaline and acid decomposition. Conclusion. 1) HPLC method was developed and validated for the simultaneous detection and quantitation of impurities formed during the synthesis of cardiazol. 2) The method proved to be sensitive, selective, precise, linear, accurate and stability-indicating." @default.
• W3025741681 created "2020-05-21" @default.
• W3025741681 creator A5009881233 @default.
• W3025741681 creator A5026942762 @default.
• W3025741681 creator A5033303670 @default.
• W3025741681 creator A5050216767 @default.
• W3025741681 creator A5051942157 @default.
• W3025741681 creator A5062080686 @default.
• W3025741681 creator A5079568635 @default.
• W3025741681 creator A5086251972 @default.
• W3025741681 date "2020-05-13" @default.
• W3025741681 modified "2023-09-24" @default.
• W3025741681 title "HPLC method for simultaneous determination of impurities and degradation products in Cardiazol" @default.
• W3025741681 cites W2470784133 @default.
• W3025741681 cites W2781792957 @default.
• W3025741681 cites W2794174737 @default.
• W3025741681 cites W2821024029 @default.
• W3025741681 doi "https://doi.org/10.3897/pharmacia.67.e37004" @default.
• W3025741681 hasPublicationYear "2020" @default.
• W3025741681 type Work @default.
• W3025741681 sameAs 3025741681 @default.
• W3025741681 citedByCount "1" @default.
• W3025741681 countsByYear W30257416812022 @default.
• W3025741681 crossrefType "journal-article" @default.
• W3025741681 hasAuthorship W3025741681A5009881233 @default.
• W3025741681 hasAuthorship W3025741681A5026942762 @default.
• W3025741681 hasAuthorship W3025741681A5033303670 @default.
• W3025741681 hasAuthorship W3025741681A5050216767 @default.
• W3025741681 hasAuthorship W3025741681A5051942157 @default.
• W3025741681 hasAuthorship W3025741681A5062080686 @default.
• W3025741681 hasAuthorship W3025741681A5079568635 @default.
• W3025741681 hasAuthorship W3025741681A5086251972 @default.
• W3025741681 hasBestOaLocation W30257416811 @default.
• W3025741681 hasConcept C113196181 @default.
• W3025741681 hasConcept C178790620 @default.
• W3025741681 hasConcept C179998833 @default.
• W3025741681 hasConcept C185592680 @default.
• W3025741681 hasConcept C2777463227 @default.
• W3025741681 hasConcept C2780112151 @default.
• W3025741681 hasConcept C43617362 @default.
• W3025741681 hasConcept C71987851 @default.
• W3025741681 hasConceptScore W3025741681C113196181 @default.
• W3025741681 hasConceptScore W3025741681C178790620 @default.
• W3025741681 hasConceptScore W3025741681C179998833 @default.
• W3025741681 hasConceptScore W3025741681C185592680 @default.
• W3025741681 hasConceptScore W3025741681C2777463227 @default.
• W3025741681 hasConceptScore W3025741681C2780112151 @default.
• W3025741681 hasConceptScore W3025741681C43617362 @default.
• W3025741681 hasConceptScore W3025741681C71987851 @default.
• W3025741681 hasIssue "1" @default.
• W3025741681 hasLocation W30257416811 @default.
• W3025741681 hasLocation W30257416812 @default.
• W3025741681 hasOpenAccess W3025741681 @default.
• W3025741681 hasPrimaryLocation W30257416811 @default.
• W3025741681 hasRelatedWork W1964989812 @default.
• W3025741681 hasRelatedWork W1979512650 @default.
• W3025741681 hasRelatedWork W2010468191 @default.
• W3025741681 hasRelatedWork W2051351267 @default.
• W3025741681 hasRelatedWork W2065520730 @default.
• W3025741681 hasRelatedWork W2107172911 @default.
• W3025741681 hasRelatedWork W2119552130 @default.
• W3025741681 hasRelatedWork W2282687875 @default.
• W3025741681 hasRelatedWork W2385809749 @default.
• W3025741681 hasRelatedWork W294862085 @default.
• W3025741681 hasVolume "67" @default.
• W3025741681 isParatext "false" @default.
• W3025741681 isRetracted "false" @default.
• W3025741681 magId "3025741681" @default.
• W3025741681 workType "article" @default.