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• W3025803602 abstract "Objective: To investigate the effects of long non-coding RNA HULC on downstream related targets regulating the migration and invasion of hepatoma cells and theirs mechanism of action. Methods: The expression of highly upregulated in liver cancer (HULC) in hepatocellular carcinoma, and adjacent normal liver tissues and different hepatocellular carcinoma cells were detected by qPCR. The correlation between clinicopathological data of HULC and liver cancer patients were analyzed. Dual-luciferase reporter gene detected the interaction between HULC and miR-186. CCK-8 assay was used to detect the effect of HULC on proliferation of hepatocellular carcinoma cells. The change in hepatocellular carcinoma cell invasions ability after HULC inhibition was detected by transwell invasion assay and migration ability after inhibition of HULC was assessed by scratch assay. Differences between groups were compared using one-way ANOVA. P < 0.05 was considered statistically significant. Results: Compared with adjacent normal liver tissue, the expression of HULC in hepatocellular carcinoma was significantly higher [(1.79 ± 0.25) vs. (0.23 ± 0.05), P < 0.05]. The expression level of HULC was highest in hepatocellular carcinoma HepG3 cells. HULC specifically banded to the 3'UTR of miR-186 and regulated the expressional activity of miR-186. After inhibiting the expression of HULC, the proliferation of hepatocellular carcinoma cells was 72 h (0.35 ± 0.09) vs. (0.82 ± 0.16), P < 0.05; 96 h (0.42 ± 0.08) vs.(1.28 ± 0.19), P < 0.05), and the ability of migration and invasion was relatively decreased in 24 h (11.2% ± 1.6%) vs. (23.5% ± 3.6%), P < 0.05; 48 h (18.6% ± 3.0%) vs. (38.6% ± 5.6%), P < 0.05; 72 h (43.6% ± 5.3% ) vs. (69.6% ± 7.6%), P < 0.05]. After inhibiting the expression of HULC, the tumor volume and body weight of tumor-bearing mice were significantly reduced [volume (2.89 ± 0.29) cm(3) vs. (0.89 ± 0.18) cm(3), P < 0.05, body weight (3.18 ± 0.41) g vs. (0.45 ± 0.09) g, P < 0.05]. Conclusion: HULC plays an important role in the occurrence and development of hepatocellular carcinoma and can influence the biological behavior of hepatoma cells by regulating the expression of downstream-related targets.目的： 探讨长链非编码RNA HULC影响下游相关靶点调控肝癌细胞的迁移和侵袭行为及其作用机制。 方法： qPCR检测肝癌和癌旁正常肝组织、不同肝癌细胞中HULC的表达情况；分析HULC和肝癌患者的临床病理资料之间的关联；双荧光素酶实验报告基因检测HULC与miR-186的相互作用；细胞计数试剂盒-8细胞增殖实验检测HULC对肝癌细胞的增殖能力的影响；Transwell侵袭实验检测抑制HULC后肝癌细胞侵袭能力的变化；划痕实验检测抑制HULC后肝癌细胞迁移能力的变化；裸鼠皮下成瘤实验检测抑制HULC后肝癌细胞体外成瘤体积和质量变化。用单因素方差分析比较组间差异。P < 0.05为差异有统计学意义。 结果： 与癌旁正常肝组织相比，肝癌组织中HULC表达明显增高[（0.23±0.05）比（1.79±0.25），P < 0.05]；肝癌细胞HepG3中HULC的表达水平最高；HULC能与miR-186的3'UTR特异性结合，调控miR-186的表达活性；抑制HULC的表达后肝癌细胞增殖[72 h（0.82±0.16）比（0.35±0.09），P < 0.05；96 h（1.28±0.19）比（0.42±0.08），P < 0.05]、迁移和侵袭的能力相对减弱[24 h（23.5%±3.6%）比（11.2%±1.6%），P < 0.05；48 h（38.6%±5.6%）比（18.6%±3.0%），P < 0.05；72 h（69.6%±7.6%）比（43.6%±5.3%），P < 0.05]；抑制HULC的表达后，荷瘤小鼠的肿瘤体积和体质量都明显减小[体积（2.89±0.29）cm(3)比（0.89±0.18）cm(3)，P < 0.05；体质量（3.18±0.41）g比（0.45±0.09)g，P < 0.05]。 结论： HULC在肝癌的发生和发展过程中起重要作用，可以通过调节下游相关靶点的表达影响肝癌细胞的生物学行为。." @default.
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• W3025803602 title "[Long non-coding RNA HULC affects downstream-related targets to regulate migration and invasion of hepatoma cells]." @default.
• W3025803602 doi "https://doi.org/10.3760/cma.j.issn.1007-3418.2018.07.007" @default.
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