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- W3025892334 abstract "Objective To investigate the effect of estradiol on myocardial voltage-gated calcium channel α1C subunit (CACNA1C) in septic mice. Methods Estradiol benzoate was injected subcutaneously into male mice followed by sepsis induced by cecal ligation and puncture (CLP). The experimental mice were divided into 4 groups: sepsis group, estradiol-treated sepsis group, sham group, estradiol-treated sham group. 3, 6, 12, 24 hours after CLP, the mRNA level of CACNA1C in left ventricular was detected by real-time fluorescent quantitative PCR, the protein expression level of CACNA1C was detected by Western blot analysis. The expression and distribution of CACNA1C were detected by immunohistochemical staining at 12 hours after CLP. Results The expression of CACNA1C was significantly decreased in sepsis group at 6 hours and remained at this low level at 12, 24 hours. The expression of CACNA1C in estradiol-treated sepsis group did not change at 3, 6 and 12 hours, but was significantly decreased at 24 hours. Conclusion Exogenous estradiol can increase the left ventricular level of CACNA1C in septic mice." @default.
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- W3025892334 date "2018-10-01" @default.
- W3025892334 modified "2023-09-27" @default.
- W3025892334 title "[Estradiol increases the level of myocardial voltage-gated calcium channel α1C subunit (CACNA1C) in septic mice]." @default.
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