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W3025953586 endingPage "9301" @default.
W3025953586 startingPage "9285" @default.
W3025953586 abstract "Voltage sensitive fluorescent dyes (VSDs) are important tools for probing signal transduction in neurons and other excitable cells. The impact of these highly lipophilic sensors has, however, been limited due to the lack of cell-specific targeting methods in brain tissue or living animals. We address this key challenge by introducing a nongenetic molecular platform for cell- and molecule-specific targeting of synthetic VSDs in the brain. We employ a dextran polymer particle to overcome the inherent lipophilicity of VSDs by dynamic encapsulation and high-affinity ligands to target the construct to specific neuronal cells utilizing only native components of the neurotransmission machinery at physiological expression levels. Dichloropane, a monoamine transporter ligand, enables targeting of dense dopaminergic axons in the mouse striatum and sparse noradrenergic axons in the mouse cortex in acute brain slices. PFQX in conjunction with ligand-directed acyl imidazole chemistry enables covalent labeling of AMPA-type glutamate receptors in the same brain regions. Probe variants bearing either a classical electrochromic ANEP dye or state-of-the-art VoltageFluor-type dye respond to membrane potential changes in a similar manner to the parent dyes, as shown by whole-cell patch recording. We demonstrate the feasibility of optical voltage recording with our probes in brain tissue with one-photon and two-photon fluorescence microscopy and define the signal limits of optical voltage imaging with synthetic sensors under a low photon budget determined by the native expression levels of the target proteins. This work demonstrates the feasibility of a chemical targeting approach and expands the possibilities of cell-specific imaging and pharmacology." @default.
W3025953586 created "2020-05-21" @default.
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W3025953586 date "2020-05-12" @default.
W3025953586 modified "2023-10-16" @default.
W3025953586 title "Chemical Targeting of Voltage Sensitive Dyes to Specific Cells and Molecules in the Brain" @default.
W3025953586 cites W1053287285 @default.
W3025953586 cites W1483210951 @default.
W3025953586 cites W1586953090 @default.
W3025953586 cites W1605717451 @default.
W3025953586 cites W1756587501 @default.
W3025953586 cites W1884375048 @default.
W3025953586 cites W1976076848 @default.
W3025953586 cites W1980397834 @default.
W3025953586 cites W1997578476 @default.
W3025953586 cites W2000300989 @default.
W3025953586 cites W2004121673 @default.
W3025953586 cites W2009201124 @default.
W3025953586 cites W2011921884 @default.
W3025953586 cites W2013631479 @default.
W3025953586 cites W2019475363 @default.
W3025953586 cites W2019653353 @default.
W3025953586 cites W2022292972 @default.
W3025953586 cites W2028029922 @default.
W3025953586 cites W2031220002 @default.
W3025953586 cites W2042572088 @default.
W3025953586 cites W2045571228 @default.
W3025953586 cites W2054466443 @default.
W3025953586 cites W2062623595 @default.
W3025953586 cites W2063637335 @default.
W3025953586 cites W2072440625 @default.
W3025953586 cites W2078501781 @default.
W3025953586 cites W2080640632 @default.
W3025953586 cites W2083858798 @default.
W3025953586 cites W2086028774 @default.
W3025953586 cites W2086244317 @default.
W3025953586 cites W2087408372 @default.
W3025953586 cites W2088807989 @default.
W3025953586 cites W2095410507 @default.
W3025953586 cites W2098322385 @default.
W3025953586 cites W2099940899 @default.
W3025953586 cites W2102979750 @default.
W3025953586 cites W2106495485 @default.
W3025953586 cites W2123337843 @default.
W3025953586 cites W2123491442 @default.
W3025953586 cites W2125336907 @default.
W3025953586 cites W2129495111 @default.
W3025953586 cites W2134178604 @default.
W3025953586 cites W2138526907 @default.
W3025953586 cites W2141260882 @default.
W3025953586 cites W2142768012 @default.
W3025953586 cites W2154616351 @default.
W3025953586 cites W2162016003 @default.
W3025953586 cites W2168913529 @default.
W3025953586 cites W2287361610 @default.
W3025953586 cites W2302615100 @default.
W3025953586 cites W2403944530 @default.
W3025953586 cites W2435050829 @default.
W3025953586 cites W2437954370 @default.
W3025953586 cites W2484021281 @default.
W3025953586 cites W2484448248 @default.
W3025953586 cites W2557384257 @default.
W3025953586 cites W2560526835 @default.
W3025953586 cites W2592758104 @default.
W3025953586 cites W2604646706 @default.
W3025953586 cites W2604969293 @default.
W3025953586 cites W2607220241 @default.
W3025953586 cites W2610074214 @default.
W3025953586 cites W2620286813 @default.
W3025953586 cites W2735247561 @default.
W3025953586 cites W2736996438 @default.
W3025953586 cites W2766096933 @default.
W3025953586 cites W2769977504 @default.
W3025953586 cites W2787386174 @default.
W3025953586 cites W2792406348 @default.
W3025953586 cites W2882976117 @default.
W3025953586 cites W2891463409 @default.
W3025953586 cites W2895238078 @default.
W3025953586 cites W2908038781 @default.
W3025953586 cites W2908861291 @default.
W3025953586 cites W2925058604 @default.
W3025953586 cites W2949289995 @default.
W3025953586 cites W2949601105 @default.
W3025953586 cites W2964363746 @default.
W3025953586 cites W2995091889 @default.