FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3026217525> ?p ?o ?g. }

• W3026217525 abstract "The putative non-ribosomal peptide synthetase (NRPS) gene cluster encoding the biosynthesis of the bioactive cyclohexapeptide thermoactinoamide A (1) was identified in Thermoactinomyces vulgaris DSM 43016. Based on in silico prediction, the biosynthetic operon was shown to contain two trimodular NRPSs, designated as ThdA and ThdB, respectively. Chemical analysis of bacterial crude extract showed the presence of thermoactinoamide A (1), thereby supporting this biosynthetic hypothesis. Notably, integrating genome mining with LC-HRMS/MS molecular networking-based investigation of the microbial metabolome, we succeeded in the identification of ten structural variants (2-11) of thermoactinoamide A (1), five of them being new compounds (thermoactinoamides G-K, 7-11). As only one thermoactinoamide operon was found in T. vulgaris, it can be assumed that all thermoactinoamide congeners are assembled by the same multimodular NRPS system. In the light of these findings, we suggest that the thermoactinoamide synthetase is able to create chemical diversity, combining the relaxed substrate selectivity of some adenylation domains with the iterative and/or alternative use of specific modules. In the frame of our screening program to discover antitumor natural products, thermoactinoamide A (1) unveiled to exert a moderate growth-inhibitory effect in BxPC-3 cancer cells in the low micromolar range, while being inactive in PANC-1 and 3AB-OS solid tumour models." @default.
• W3026217525 created "2020-05-29" @default.
• W3026217525 creator A5012587031 @default.
• W3026217525 creator A5015843701 @default.
• W3026217525 creator A5059726492 @default.
• W3026217525 creator A5088863031 @default.
• W3026217525 date "2020-05-21" @default.
• W3026217525 modified "2023-10-18" @default.
• W3026217525 title "Identification of the Biosynthetic Gene Cluster of Thermoactinoamides and Discovery of New Congeners by Integrated Genome Mining and MS-Based Molecular Networking" @default.
• W3026217525 cites W1941607253 @default.
• W3026217525 cites W1968248059 @default.
• W3026217525 cites W1972237830 @default.
• W3026217525 cites W1981892047 @default.
• W3026217525 cites W1989299822 @default.
• W3026217525 cites W2012960603 @default.
• W3026217525 cites W2018849951 @default.
• W3026217525 cites W2047634157 @default.
• W3026217525 cites W2050857499 @default.
• W3026217525 cites W2055043387 @default.
• W3026217525 cites W2066182578 @default.
• W3026217525 cites W2080895748 @default.
• W3026217525 cites W2085677240 @default.
• W3026217525 cites W2104696872 @default.
• W3026217525 cites W2111689333 @default.
• W3026217525 cites W2114839447 @default.
• W3026217525 cites W2131887397 @default.
• W3026217525 cites W2153812990 @default.
• W3026217525 cites W2159675211 @default.
• W3026217525 cites W2159983165 @default.
• W3026217525 cites W2163111540 @default.
• W3026217525 cites W2326842020 @default.
• W3026217525 cites W2340169167 @default.
• W3026217525 cites W2410975458 @default.
• W3026217525 cites W2429635451 @default.
• W3026217525 cites W2504691963 @default.
• W3026217525 cites W2529422320 @default.
• W3026217525 cites W2594267091 @default.
• W3026217525 cites W2608736608 @default.
• W3026217525 cites W2609551972 @default.
• W3026217525 cites W2616993478 @default.
• W3026217525 cites W2740856177 @default.
• W3026217525 cites W2748055317 @default.
• W3026217525 cites W2806022215 @default.
• W3026217525 cites W2895532240 @default.
• W3026217525 cites W2914366990 @default.
• W3026217525 cites W2922462915 @default.
• W3026217525 cites W2939116319 @default.
• W3026217525 cites W2943963358 @default.
• W3026217525 cites W2958196293 @default.
• W3026217525 cites W2979613280 @default.
• W3026217525 cites W2980680496 @default.
• W3026217525 doi "https://doi.org/10.3389/fchem.2020.00397" @default.
• W3026217525 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7253712" @default.
• W3026217525 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32528927" @default.
• W3026217525 hasPublicationYear "2020" @default.
• W3026217525 type Work @default.
• W3026217525 sameAs 3026217525 @default.
• W3026217525 citedByCount "9" @default.
• W3026217525 countsByYear W30262175252020 @default.
• W3026217525 countsByYear W30262175252021 @default.
• W3026217525 countsByYear W30262175252022 @default.
• W3026217525 countsByYear W30262175252023 @default.
• W3026217525 crossrefType "journal-article" @default.
• W3026217525 hasAuthorship W3026217525A5012587031 @default.
• W3026217525 hasAuthorship W3026217525A5015843701 @default.
• W3026217525 hasAuthorship W3026217525A5059726492 @default.
• W3026217525 hasAuthorship W3026217525A5088863031 @default.
• W3026217525 hasBestOaLocation W30262175251 @default.
• W3026217525 hasConcept C104317684 @default.
• W3026217525 hasConcept C116834253 @default.
• W3026217525 hasConcept C135870905 @default.
• W3026217525 hasConcept C141231307 @default.
• W3026217525 hasConcept C203075996 @default.
• W3026217525 hasConcept C2775905019 @default.
• W3026217525 hasConcept C2777477808 @default.
• W3026217525 hasConcept C36857842 @default.
• W3026217525 hasConcept C54355233 @default.
• W3026217525 hasConcept C547475151 @default.
• W3026217525 hasConcept C553450214 @default.
• W3026217525 hasConcept C55493867 @default.
• W3026217525 hasConcept C59822182 @default.
• W3026217525 hasConcept C70721500 @default.
• W3026217525 hasConcept C74187038 @default.
• W3026217525 hasConcept C86803240 @default.
• W3026217525 hasConceptScore W3026217525C104317684 @default.
• W3026217525 hasConceptScore W3026217525C116834253 @default.
• W3026217525 hasConceptScore W3026217525C135870905 @default.
• W3026217525 hasConceptScore W3026217525C141231307 @default.
• W3026217525 hasConceptScore W3026217525C203075996 @default.
• W3026217525 hasConceptScore W3026217525C2775905019 @default.
• W3026217525 hasConceptScore W3026217525C2777477808 @default.
• W3026217525 hasConceptScore W3026217525C36857842 @default.
• W3026217525 hasConceptScore W3026217525C54355233 @default.
• W3026217525 hasConceptScore W3026217525C547475151 @default.
• W3026217525 hasConceptScore W3026217525C553450214 @default.
• W3026217525 hasConceptScore W3026217525C55493867 @default.
• W3026217525 hasConceptScore W3026217525C59822182 @default.
• W3026217525 hasConceptScore W3026217525C70721500 @default.
• W3026217525 hasConceptScore W3026217525C74187038 @default.
• W3026217525 hasConceptScore W3026217525C86803240 @default.

• next