FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3026521310> ?p ?o ?g. }

• W3026521310 endingPage "8208" @default.
• W3026521310 startingPage "8201" @default.
• W3026521310 abstract "The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis. In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients. Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS. Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera. Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra. Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples. We found that our strategy was able to distinguish isomers of glycopeptides where several isomers showed distinctly different patterns between cirrhosis and HCC patients. We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9. Further analysis showed that the difference may be related to the sialic acid/galactose linkage of the glycan motif." @default.
• W3026521310 created "2020-05-29" @default.
• W3026521310 creator A5016524935 @default.
• W3026521310 creator A5018596386 @default.
• W3026521310 creator A5069522797 @default.
• W3026521310 creator A5074120071 @default.
• W3026521310 creator A5078663555 @default.
• W3026521310 date "2020-05-19" @default.
• W3026521310 modified "2023-10-04" @default.
• W3026521310 title "Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS" @default.
• W3026521310 cites W1182461782 @default.
• W3026521310 cites W1965262096 @default.
• W3026521310 cites W1976484683 @default.
• W3026521310 cites W1991787417 @default.
• W3026521310 cites W2020265320 @default.
• W3026521310 cites W2041498352 @default.
• W3026521310 cites W2064347820 @default.
• W3026521310 cites W2068980146 @default.
• W3026521310 cites W2083966880 @default.
• W3026521310 cites W2105167594 @default.
• W3026521310 cites W2110438218 @default.
• W3026521310 cites W2136504046 @default.
• W3026521310 cites W2141091417 @default.
• W3026521310 cites W2171521392 @default.
• W3026521310 cites W2247651258 @default.
• W3026521310 cites W2421415236 @default.
• W3026521310 cites W2442134451 @default.
• W3026521310 cites W2501817683 @default.
• W3026521310 cites W2607304586 @default.
• W3026521310 cites W2736206659 @default.
• W3026521310 cites W2752557010 @default.
• W3026521310 cites W2790239670 @default.
• W3026521310 cites W2810738034 @default.
• W3026521310 cites W2896305731 @default.
• W3026521310 cites W2901013713 @default.
• W3026521310 cites W2904617557 @default.
• W3026521310 cites W2914583580 @default.
• W3026521310 cites W2943939024 @default.
• W3026521310 cites W2965045484 @default.
• W3026521310 cites W2972836538 @default.
• W3026521310 cites W2999924529 @default.
• W3026521310 doi "https://doi.org/10.1021/acs.analchem.0c00420" @default.
• W3026521310 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7373126" @default.
• W3026521310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32426967" @default.
• W3026521310 hasPublicationYear "2020" @default.
• W3026521310 type Work @default.
• W3026521310 sameAs 3026521310 @default.
• W3026521310 citedByCount "18" @default.
• W3026521310 countsByYear W30265213102020 @default.
• W3026521310 countsByYear W30265213102021 @default.
• W3026521310 countsByYear W30265213102022 @default.
• W3026521310 countsByYear W30265213102023 @default.
• W3026521310 crossrefType "journal-article" @default.
• W3026521310 hasAuthorship W3026521310A5016524935 @default.
• W3026521310 hasAuthorship W3026521310A5018596386 @default.
• W3026521310 hasAuthorship W3026521310A5069522797 @default.
• W3026521310 hasAuthorship W3026521310A5074120071 @default.
• W3026521310 hasAuthorship W3026521310A5078663555 @default.
• W3026521310 hasBestOaLocation W30265213102 @default.
• W3026521310 hasConcept C108625454 @default.
• W3026521310 hasConcept C126322002 @default.
• W3026521310 hasConcept C137610392 @default.
• W3026521310 hasConcept C156534840 @default.
• W3026521310 hasConcept C162356407 @default.
• W3026521310 hasConcept C185592680 @default.
• W3026521310 hasConcept C206212055 @default.
• W3026521310 hasConcept C2777214474 @default.
• W3026521310 hasConcept C2777313579 @default.
• W3026521310 hasConcept C31827203 @default.
• W3026521310 hasConcept C43617362 @default.
• W3026521310 hasConcept C501593827 @default.
• W3026521310 hasConcept C55493867 @default.
• W3026521310 hasConcept C64489805 @default.
• W3026521310 hasConcept C71924100 @default.
• W3026521310 hasConceptScore W3026521310C108625454 @default.
• W3026521310 hasConceptScore W3026521310C126322002 @default.
• W3026521310 hasConceptScore W3026521310C137610392 @default.
• W3026521310 hasConceptScore W3026521310C156534840 @default.
• W3026521310 hasConceptScore W3026521310C162356407 @default.
• W3026521310 hasConceptScore W3026521310C185592680 @default.
• W3026521310 hasConceptScore W3026521310C206212055 @default.
• W3026521310 hasConceptScore W3026521310C2777214474 @default.
• W3026521310 hasConceptScore W3026521310C2777313579 @default.
• W3026521310 hasConceptScore W3026521310C31827203 @default.
• W3026521310 hasConceptScore W3026521310C43617362 @default.
• W3026521310 hasConceptScore W3026521310C501593827 @default.
• W3026521310 hasConceptScore W3026521310C55493867 @default.
• W3026521310 hasConceptScore W3026521310C64489805 @default.
• W3026521310 hasConceptScore W3026521310C71924100 @default.
• W3026521310 hasFunder F4320321001 @default.
• W3026521310 hasFunder F4320337351 @default.
• W3026521310 hasIssue "12" @default.
• W3026521310 hasLocation W30265213101 @default.
• W3026521310 hasLocation W30265213102 @default.
• W3026521310 hasOpenAccess W3026521310 @default.
• W3026521310 hasPrimaryLocation W30265213101 @default.
• W3026521310 hasRelatedWork W1876022371 @default.
• W3026521310 hasRelatedWork W1935496402 @default.

• next