FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3026534189> ?p ?o ?g. }

• W3026534189 endingPage "109125" @default.
• W3026534189 startingPage "109125" @default.
• W3026534189 abstract "Prevention of infection with canine heartworm (Dirofilaria immitis) is based on the compliant administration of macrocyclic lactone (ML) drugs. Resistance to ML drugs is well documented in D. immitis; however, there remains a paucity of information on the spatial distribution and prevalence of resistant isolates. This project aims to improve understanding of ML-resistance by using a population genetic approach. We developed a large panel of microsatellite loci and identified 12 novel highly polymorphic markers. These 12, and five previously published markers were used to screen pools of microfilariae from 16 confirmed drug-susceptible, 25 confirmed drug-resistant, and from 10 suspected drug-resistant field isolates. In isolates where microfilarial suppression testing indicated resistance, Spatial Principal Component Analysis (sPCoA), Neighbor Joining Trees and Bayesian clustering all revealed high genetic similarity between pre- and post-treatment samples. Somewhat surprisingly, the Neighbor Joining tree and sPCoA generated using pairwise Nei’s distances did not reveal clustering for resistant isolates, nor did it reveal state-level geographic clustering from samples collected in Georgia, Louisiana or Mississippi. In contrast, Discriminant Analysis of Principle Components was able to discriminate between susceptible, suspected-resistant and resistant samples. However, no resistance-associated markers were detected, and this clustering was driven by the combined effects of multiple alleles across multiple loci. Additionally, we measured unexpectedly large genetic distances between different passages of laboratory strains that originated from the same source infection. This finding strongly suggests that the genetic makeup of laboratory isolates can change substantially with each passage, likely due to genetic bottlenecking. Taken together, these data suggest greater than expected genetic variability in the resistant isolates, and in D. immitis overall. Our results also suggest that microsatellite genotyping lacks the sensitivity to detect a specific genetic signature for resistance. Future investigations using genomic analyses will be required to elucidate the genetic relationships of ML-resistant isolates." @default.
• W3026534189 created "2020-05-29" @default.
• W3026534189 creator A5009022906 @default.
• W3026534189 creator A5021384133 @default.
• W3026534189 creator A5047523838 @default.
• W3026534189 creator A5053039267 @default.
• W3026534189 creator A5058811797 @default.
• W3026534189 creator A5081698117 @default.
• W3026534189 creator A5085984380 @default.
• W3026534189 date "2020-07-01" @default.
• W3026534189 modified "2023-10-01" @default.
• W3026534189 title "Using population genetics to examine relationships of Dirofilaria immitis based on both macrocyclic lactone-resistance status and geography" @default.
• W3026534189 cites W1672683010 @default.
• W3026534189 cites W1871123198 @default.
• W3026534189 cites W1977601452 @default.
• W3026534189 cites W2019303799 @default.
• W3026534189 cites W2019454741 @default.
• W3026534189 cites W2019478436 @default.
• W3026534189 cites W2019603369 @default.
• W3026534189 cites W2034337979 @default.
• W3026534189 cites W2035839217 @default.
• W3026534189 cites W2040840128 @default.
• W3026534189 cites W2043575878 @default.
• W3026534189 cites W2050040700 @default.
• W3026534189 cites W2051036114 @default.
• W3026534189 cites W2077740759 @default.
• W3026534189 cites W2095182829 @default.
• W3026534189 cites W2098126593 @default.
• W3026534189 cites W2099201605 @default.
• W3026534189 cites W2100849511 @default.
• W3026534189 cites W2106437798 @default.
• W3026534189 cites W2114750701 @default.
• W3026534189 cites W2116585889 @default.
• W3026534189 cites W2127890421 @default.
• W3026534189 cites W2128880918 @default.
• W3026534189 cites W2142644281 @default.
• W3026534189 cites W2146290595 @default.
• W3026534189 cites W2151409320 @default.
• W3026534189 cites W2155670634 @default.
• W3026534189 cites W2161339576 @default.
• W3026534189 cites W2162050968 @default.
• W3026534189 cites W2170050310 @default.
• W3026534189 cites W2267484242 @default.
• W3026534189 cites W2443528581 @default.
• W3026534189 cites W2767403507 @default.
• W3026534189 cites W2767545190 @default.
• W3026534189 cites W2767691815 @default.
• W3026534189 cites W2767708888 @default.
• W3026534189 cites W2767971152 @default.
• W3026534189 cites W2884048087 @default.
• W3026534189 doi "https://doi.org/10.1016/j.vetpar.2020.109125" @default.
• W3026534189 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32535487" @default.
• W3026534189 hasPublicationYear "2020" @default.
• W3026534189 type Work @default.
• W3026534189 sameAs 3026534189 @default.
• W3026534189 citedByCount "12" @default.
• W3026534189 countsByYear W30265341892020 @default.
• W3026534189 countsByYear W30265341892021 @default.
• W3026534189 countsByYear W30265341892022 @default.
• W3026534189 countsByYear W30265341892023 @default.
• W3026534189 crossrefType "journal-article" @default.
• W3026534189 hasAuthorship W3026534189A5009022906 @default.
• W3026534189 hasAuthorship W3026534189A5021384133 @default.
• W3026534189 hasAuthorship W3026534189A5047523838 @default.
• W3026534189 hasAuthorship W3026534189A5053039267 @default.
• W3026534189 hasAuthorship W3026534189A5058811797 @default.
• W3026534189 hasAuthorship W3026534189A5081698117 @default.
• W3026534189 hasAuthorship W3026534189A5085984380 @default.
• W3026534189 hasBestOaLocation W30265341892 @default.
• W3026534189 hasConcept C104317684 @default.
• W3026534189 hasConcept C114851261 @default.
• W3026534189 hasConcept C138592617 @default.
• W3026534189 hasConcept C144024400 @default.
• W3026534189 hasConcept C149923435 @default.
• W3026534189 hasConcept C165901193 @default.
• W3026534189 hasConcept C180754005 @default.
• W3026534189 hasConcept C2776355835 @default.
• W3026534189 hasConcept C2908647359 @default.
• W3026534189 hasConcept C42972112 @default.
• W3026534189 hasConcept C54355233 @default.
• W3026534189 hasConcept C61320498 @default.
• W3026534189 hasConcept C68873052 @default.
• W3026534189 hasConcept C71924100 @default.
• W3026534189 hasConcept C78458016 @default.
• W3026534189 hasConcept C86803240 @default.
• W3026534189 hasConcept C90856448 @default.
• W3026534189 hasConceptScore W3026534189C104317684 @default.
• W3026534189 hasConceptScore W3026534189C114851261 @default.
• W3026534189 hasConceptScore W3026534189C138592617 @default.
• W3026534189 hasConceptScore W3026534189C144024400 @default.
• W3026534189 hasConceptScore W3026534189C149923435 @default.
• W3026534189 hasConceptScore W3026534189C165901193 @default.
• W3026534189 hasConceptScore W3026534189C180754005 @default.
• W3026534189 hasConceptScore W3026534189C2776355835 @default.
• W3026534189 hasConceptScore W3026534189C2908647359 @default.
• W3026534189 hasConceptScore W3026534189C42972112 @default.
• W3026534189 hasConceptScore W3026534189C54355233 @default.
• W3026534189 hasConceptScore W3026534189C61320498 @default.

• next