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- W3026552511 abstract "Background & Aim Cells might be damaged by environmental changes during the freezing process. There are various factors that influence the function of cells cultured after cryopreservation and thawing. Among cryopreservation solutions, cryoprotective agents such as glycerol, ethylene glycol, and dimethyl sulfoxide (DMSO) are the most effective due to their high rate of penetration into cells. In this study, we investigated the effects of long-term storage (7 years in liquid nitrogen phase) on the characteristics of equine adipose tissues-derived mesenchymal stem cells (eAD-MSCs). Methods, Results & Conclusion We examined the cell viability of cryopreserved eAD-MSCs (10%, DMSO, 40% FBS, 50% culture media) in liquid nitrogen phase for 7 years. Next, we investigated the cellular morphology, proliferating capacities, expression of cell surface markers such as CD13, CD34, CD44, CD45, CD90, and CD105, mesodermal differentiation potentials, and expression of senescence-related markers of p53, p21, and telomerase reverse transcriptase in eAD-MSCs after cryopreservation. The eAD-MSCs were analyzed immediately and after being frozen in liquid nitrogen for 1 year ( 7 years, B), respectively. After cryopreservation for 1∼7 years, B eAD-MSCs were similar their cellular morphology, proliferating capacities, and expression of cell surface markers, compared with A eAD-MSCs. Also, cryopreservation did not affect the adipogenic, chondrogenic, or osteogenic differentiation potentials of A and B eAD-MSCs. In this study, cryopreservation for (or over) 7 years maintain the stem cell phenotype and differentiation potentials of eAD-MSCs. These results will be an advantage that can be effectively used for future development of cell-based therapies." @default.
- W3026552511 created "2020-05-29" @default.
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- W3026552511 date "2020-05-01" @default.
- W3026552511 modified "2023-10-18" @default.
- W3026552511 title "Stemness of cryopreserved animal adult stem cells for 7 years" @default.
- W3026552511 doi "https://doi.org/10.1016/j.jcyt.2020.03.065" @default.
- W3026552511 hasPublicationYear "2020" @default.
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