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- W3026616153 abstract "Changes in NAD (or NADP) reduction due to microelectrophoretic additions of metabolites are followed in localized areas of single living cells (EL2 cells) by means of a modified Chance-Legallais microfluorimeter or an optimized photon counting method with a time resolution from 1 to 1 100 second. The glycolytic activity is dependent upon mitochondrial-extramitochondrial competition for activators and the removal of reducing equivalents. At 23° the glycolytic rate is decreased by several times in presence of lactate, with recovery at 37°. While the LDH pathway is predominant, the contributions of the mitochondrial oxidations and a-glycerophosphate shuttle are also significant. In rapid microfluorimetric recordings the initial rate of NAD reduction can be made to vary with substrate concentration or changes in metabolites and cell type." @default.
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- W3026616153 date "1972-01-01" @default.
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- W3026616153 title "KINETICS OF ENZYME-SUBSTRATE REACTIONS IN SINGLE LIVING CELLS" @default.
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- W3026616153 doi "https://doi.org/10.1016/b978-0-08-016874-6.50066-7" @default.
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