FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3026630659> ?p ?o ?g. }

• W3026630659 endingPage "S47" @default.
• W3026630659 startingPage "S47" @default.
• W3026630659 abstract "Background & Aim Wharton's Jelly Mesenchymal Stem Cell (WJMSCs)-derived exosomes have been studied extensively in animal models of degenerative and chronic diseases. Thus, the development of a large-scale manufacturing procedure to harvest clinical-grade exosomes is required for the translation of exosome to the clinic. We have developed an exosome production technique to manufacture large quantities of exosomes using the quantum bioreactor (Terumo BCT) with both fetal bovine serum (FBS) and human platelet lysate (hPLT) supplemented expansion media. AWe aimed to compare the miRNA cargo of exosomes isolated from FBS-expanded and PLT-expanded WJMSC to determine if growth media is a significant contributor to exosome product preparation. Methods, Results & Conclusion WJMSCs were isolated from Wharton's Jelly and expanded to passage 1. After harvest, 30 × 106 cells were loaded into the quantum bioreactor and expanded for 1 week to achieve maximum confluence. Expansion media was washed and replaced with serum-free aMEM media. Conditioned media was collected every 24 hours for 120 hours and exosomes were precipitated by sequential centrifugation and ultracentrifugation. NanoSight analysis of the final products revealed a production of 15.0 × 1012± 0.32 × 1012 total particles with an average mode size of 111±4.78 nm. Surface protein analysis revealed positive expression of exosome markers CD9, CD63, and CD81. Exosome products were subjected to miRNA isolation, sequencing, and miRNA identification for bioinformatics analysis with the Qiagen IPA software. miRNA analysis was completed using 3 WJMSC donors grown in 20% FBS or 5%PLT supplemented media. Sequencing results identified 157 miRNAs in the PLT group and 154 miRNAs in the FBS group (with a 100-copy minimum expression cut off). FBS and PLT comparison revealed 142 common miRNAs with 15 unique to PLT and 12 unique to FBS. Further RNA target analysis revealed 68 miRNAs that target 1024 mRNA in the PLT group and 64 miRNA that target 1048 mRNA in the FBS group. The two groups share 998 common mRNA targets with 27 unique to PLT and 51 unique to FBS. Canonical pathway analysis of the unique targets reveals the top pathway hits, serotonin receptor and JAK/Stat signaling for PLT and IL-7 and RANK signaling for FBS. We found both expansion medias to be effective for large scale expansion and downstream exosome production. miRNA sequencing revealed small variations in the total number of predicted mRNA targets, suggesting a conserved therapeutic effect between the two groups. Wharton's Jelly Mesenchymal Stem Cell (WJMSCs)-derived exosomes have been studied extensively in animal models of degenerative and chronic diseases. Thus, the development of a large-scale manufacturing procedure to harvest clinical-grade exosomes is required for the translation of exosome to the clinic. We have developed an exosome production technique to manufacture large quantities of exosomes using the quantum bioreactor (Terumo BCT) with both fetal bovine serum (FBS) and human platelet lysate (hPLT) supplemented expansion media. AWe aimed to compare the miRNA cargo of exosomes isolated from FBS-expanded and PLT-expanded WJMSC to determine if growth media is a significant contributor to exosome product preparation. WJMSCs were isolated from Wharton's Jelly and expanded to passage 1. After harvest, 30 × 106 cells were loaded into the quantum bioreactor and expanded for 1 week to achieve maximum confluence. Expansion media was washed and replaced with serum-free aMEM media. Conditioned media was collected every 24 hours for 120 hours and exosomes were precipitated by sequential centrifugation and ultracentrifugation. NanoSight analysis of the final products revealed a production of 15.0 × 1012± 0.32 × 1012 total particles with an average mode size of 111±4.78 nm. Surface protein analysis revealed positive expression of exosome markers CD9, CD63, and CD81. Exosome products were subjected to miRNA isolation, sequencing, and miRNA identification for bioinformatics analysis with the Qiagen IPA software. miRNA analysis was completed using 3 WJMSC donors grown in 20% FBS or 5%PLT supplemented media." @default.
• W3026630659 created "2020-05-29" @default.
• W3026630659 creator A5014439429 @default.
• W3026630659 creator A5023488496 @default.
• W3026630659 creator A5062453313 @default.
• W3026630659 creator A5091680684 @default.
• W3026630659 date "2020-05-01" @default.
• W3026630659 modified "2023-09-26" @default.
• W3026630659 title "Comparison of miRNA Cargo within Wharton's Jelly mesenchymal stem cell-derived exosomes manufactured in FBS and hPLT expansion media" @default.
• W3026630659 doi "https://doi.org/10.1016/j.jcyt.2020.03.053" @default.
• W3026630659 hasPublicationYear "2020" @default.
• W3026630659 type Work @default.
• W3026630659 sameAs 3026630659 @default.
• W3026630659 citedByCount "0" @default.
• W3026630659 crossrefType "journal-article" @default.
• W3026630659 hasAuthorship W3026630659A5014439429 @default.
• W3026630659 hasAuthorship W3026630659A5023488496 @default.
• W3026630659 hasAuthorship W3026630659A5062453313 @default.
• W3026630659 hasAuthorship W3026630659A5091680684 @default.
• W3026630659 hasConcept C104317684 @default.
• W3026630659 hasConcept C119577978 @default.
• W3026630659 hasConcept C145059251 @default.
• W3026630659 hasConcept C153911025 @default.
• W3026630659 hasConcept C16685009 @default.
• W3026630659 hasConcept C185592680 @default.
• W3026630659 hasConcept C198826908 @default.
• W3026630659 hasConcept C20518536 @default.
• W3026630659 hasConcept C2776385711 @default.
• W3026630659 hasConcept C2776662772 @default.
• W3026630659 hasConcept C2778120352 @default.
• W3026630659 hasConcept C2779404806 @default.
• W3026630659 hasConcept C2781261824 @default.
• W3026630659 hasConcept C54355233 @default.
• W3026630659 hasConcept C55493867 @default.
• W3026630659 hasConcept C71924100 @default.
• W3026630659 hasConcept C7623868 @default.
• W3026630659 hasConcept C81885089 @default.
• W3026630659 hasConcept C86803240 @default.
• W3026630659 hasConcept C95444343 @default.
• W3026630659 hasConceptScore W3026630659C104317684 @default.
• W3026630659 hasConceptScore W3026630659C119577978 @default.
• W3026630659 hasConceptScore W3026630659C145059251 @default.
• W3026630659 hasConceptScore W3026630659C153911025 @default.
• W3026630659 hasConceptScore W3026630659C16685009 @default.
• W3026630659 hasConceptScore W3026630659C185592680 @default.
• W3026630659 hasConceptScore W3026630659C198826908 @default.
• W3026630659 hasConceptScore W3026630659C20518536 @default.
• W3026630659 hasConceptScore W3026630659C2776385711 @default.
• W3026630659 hasConceptScore W3026630659C2776662772 @default.
• W3026630659 hasConceptScore W3026630659C2778120352 @default.
• W3026630659 hasConceptScore W3026630659C2779404806 @default.
• W3026630659 hasConceptScore W3026630659C2781261824 @default.
• W3026630659 hasConceptScore W3026630659C54355233 @default.
• W3026630659 hasConceptScore W3026630659C55493867 @default.
• W3026630659 hasConceptScore W3026630659C71924100 @default.
• W3026630659 hasConceptScore W3026630659C7623868 @default.
• W3026630659 hasConceptScore W3026630659C81885089 @default.
• W3026630659 hasConceptScore W3026630659C86803240 @default.
• W3026630659 hasConceptScore W3026630659C95444343 @default.
• W3026630659 hasIssue "5" @default.
• W3026630659 hasLocation W30266306591 @default.
• W3026630659 hasOpenAccess W3026630659 @default.
• W3026630659 hasPrimaryLocation W30266306591 @default.
• W3026630659 hasRelatedWork W2731074560 @default.
• W3026630659 hasRelatedWork W2795057253 @default.
• W3026630659 hasRelatedWork W2907832694 @default.
• W3026630659 hasRelatedWork W2921573102 @default.
• W3026630659 hasRelatedWork W2945013784 @default.
• W3026630659 hasRelatedWork W2968935972 @default.
• W3026630659 hasRelatedWork W3009964850 @default.
• W3026630659 hasRelatedWork W3026630659 @default.
• W3026630659 hasRelatedWork W3094249498 @default.
• W3026630659 hasRelatedWork W3198772284 @default.
• W3026630659 hasVolume "22" @default.
• W3026630659 isParatext "false" @default.
• W3026630659 isRetracted "false" @default.
• W3026630659 magId "3026630659" @default.
• W3026630659 workType "article" @default.