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- W3027388204 abstract "At cell division, the mammalian kinetochore binds many spindle microtubules that make up the kinetochore-fiber. To segregate chromosomes, the kinetochore-fiber must be dynamic and generate and respond to force. Yet, how it remodels under force remains poorly understood. Kinetochore-fibers cannot be reconstituted in vitro, and exerting controlled forces in vivo remains challenging. Here, we use microneedles to pull on mammalian kinetochore-fibers and probe how sustained force regulates their dynamics and structure. We show that force lengthens kinetochore-fibers by persistently favoring plus-end polymerization, not by increasing polymerization rate. We demonstrate that force suppresses depolymerization at both plus and minus ends, rather than sliding microtubules within the kinetochore-fiber. Finally, we observe that kinetochore-fibers break but do not detach from kinetochores or poles. Together, this work suggests an engineering principle for spindle structural homeostasis: different physical mechanisms of local force dissipation by the k-fiber limit force transmission to preserve robust spindle structure. These findings may inform how other dynamic, force-generating cellular machines achieve mechanical robustness." @default.
- W3027388204 created "2020-05-29" @default.
- W3027388204 creator A5015163896 @default.
- W3027388204 creator A5051453476 @default.
- W3027388204 creator A5079338191 @default.
- W3027388204 date "2020-05-20" @default.
- W3027388204 modified "2023-10-14" @default.
- W3027388204 title "Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structure" @default.
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- W3027388204 doi "https://doi.org/10.1083/jcb.201911090" @default.
- W3027388204 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7401803" @default.
- W3027388204 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32435797" @default.
- W3027388204 hasPublicationYear "2020" @default.
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