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- W3027779714 abstract "Publisher SummaryThis chapter presents an improved method to obtain a large number of mutants in a defined region of DNA. In recent years, production of mutations in vitro has supplemented the ability to learn from naturally occurring mutations about specific genes and gene products. With oligonucleotide-directed protocols, specific individual mutations can be obtained by targeting relatively few residues of known interest without reliance on the phenotype. A significant advantage of random mutagenesis is the ability to obtain mutations at various sites and in different combinations with a single treatment. Conditions for mutagenesis of nucleic acids with sodium bisulfite have been developed based on the well-characterized reactions of this reagent with the bases of nucleosides, nucleotides, and nucleic acids. Single-stranded recombinant phage DNA is a ready target for reaction with sodium bisulfite, but the phage genes must be protected from mutagenesis because they are all essential to the phage life cycle." @default.
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- W3027779714 date "1989-01-01" @default.
- W3027779714 modified "2023-10-17" @default.
- W3027779714 title "An Improved Method to Obtain a Large Number of Mutants in a Defined Region of DNA" @default.
- W3027779714 doi "https://doi.org/10.1016/b978-0-12-765560-4.50039-3" @default.
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