Matches in SemOpenAlex for { <https://semopenalex.org/work/W3027782010> ?p ?o ?g. }
- W3027782010 endingPage "929" @default.
- W3027782010 startingPage "920" @default.
- W3027782010 abstract "The fundamental and assorted roles of β-1,3-glucans in nature are underpinned on diverse chemistry and molecular structures, demanding sophisticated and intricate enzymatic systems for their processing. In this work, the selectivity and modes of action of a glycoside hydrolase family active on β-1,3-glucans were systematically investigated combining sequence similarity network, phylogeny, X-ray crystallography, enzyme kinetics, mutagenesis and molecular dynamics. This family exhibits a minimalist and versatile (α/β)-barrel scaffold, which can harbor distinguishing exo or endo modes of action, including an ancillary-binding site for the anchoring of triple-helical β-1,3-glucans. The substrate binding occurs via a hydrophobic knuckle complementary to the canonical curved conformation of β-1,3-glucans or through a substrate conformational change imposed by the active-site topology of some fungal enzymes. Together, these findings expand our understanding of the enzymatic arsenal of bacteria and fungi for the breakdown and modification of β-1,3-glucans, which can be exploited for biotechnological applications. Comprehensive informatic, structural and biochemical characterization of the GH128 family defines subgroups of glycoside hydrolase enzymes with unique recognition and cleavage mechanisms for 1,3-beta-glucan polysaccharide substrates." @default.
- W3027782010 created "2020-05-29" @default.
- W3027782010 creator A5007455893 @default.
- W3027782010 creator A5009590779 @default.
- W3027782010 creator A5011500405 @default.
- W3027782010 creator A5012876343 @default.
- W3027782010 creator A5015306708 @default.
- W3027782010 creator A5018430103 @default.
- W3027782010 creator A5019713781 @default.
- W3027782010 creator A5036551022 @default.
- W3027782010 creator A5038331268 @default.
- W3027782010 creator A5040972025 @default.
- W3027782010 creator A5041118866 @default.
- W3027782010 creator A5054612480 @default.
- W3027782010 creator A5061723349 @default.
- W3027782010 creator A5061967753 @default.
- W3027782010 creator A5070417530 @default.
- W3027782010 creator A5073643896 @default.
- W3027782010 creator A5076332156 @default.
- W3027782010 creator A5082381783 @default.
- W3027782010 creator A5084814047 @default.
- W3027782010 date "2020-05-25" @default.
- W3027782010 modified "2023-10-18" @default.
- W3027782010 title "Structural insights into β-1,3-glucan cleavage by a glycoside hydrolase family" @default.
- W3027782010 cites W1803804183 @default.
- W3027782010 cites W1976499671 @default.
- W3027782010 cites W1987250042 @default.
- W3027782010 cites W1987370810 @default.
- W3027782010 cites W1994191943 @default.
- W3027782010 cites W2003655375 @default.
- W3027782010 cites W2003949305 @default.
- W3027782010 cites W2004642364 @default.
- W3027782010 cites W2005250838 @default.
- W3027782010 cites W2009674410 @default.
- W3027782010 cites W2011479098 @default.
- W3027782010 cites W2017389387 @default.
- W3027782010 cites W2021741281 @default.
- W3027782010 cites W2031620290 @default.
- W3027782010 cites W2038758364 @default.
- W3027782010 cites W2038840577 @default.
- W3027782010 cites W2054979768 @default.
- W3027782010 cites W2065111539 @default.
- W3027782010 cites W2065523560 @default.
- W3027782010 cites W2066054435 @default.
- W3027782010 cites W2067174909 @default.
- W3027782010 cites W2069042781 @default.
- W3027782010 cites W2089014779 @default.
- W3027782010 cites W2091034002 @default.
- W3027782010 cites W2091075510 @default.
- W3027782010 cites W2102918226 @default.
- W3027782010 cites W2105329815 @default.
- W3027782010 cites W2108248497 @default.
- W3027782010 cites W2109184569 @default.
- W3027782010 cites W2124026197 @default.
- W3027782010 cites W2131225429 @default.
- W3027782010 cites W2131350133 @default.
- W3027782010 cites W2132926880 @default.
- W3027782010 cites W2135621733 @default.
- W3027782010 cites W2135750241 @default.
- W3027782010 cites W2137292807 @default.
- W3027782010 cites W2146012412 @default.
- W3027782010 cites W2149561246 @default.
- W3027782010 cites W2150494049 @default.
- W3027782010 cites W2150981663 @default.
- W3027782010 cites W2159944520 @default.
- W3027782010 cites W2163341755 @default.
- W3027782010 cites W2168240189 @default.
- W3027782010 cites W2170264257 @default.
- W3027782010 cites W2170747616 @default.
- W3027782010 cites W2293513308 @default.
- W3027782010 cites W2503752128 @default.
- W3027782010 cites W2610364589 @default.
- W3027782010 cites W2707646777 @default.
- W3027782010 cites W2739412793 @default.
- W3027782010 cites W2743889485 @default.
- W3027782010 cites W2766775456 @default.
- W3027782010 cites W2865215684 @default.
- W3027782010 cites W2885904303 @default.
- W3027782010 cites W2899005647 @default.
- W3027782010 cites W2922258649 @default.
- W3027782010 cites W2972315562 @default.
- W3027782010 cites W31975475 @default.
- W3027782010 cites W4248872320 @default.
- W3027782010 doi "https://doi.org/10.1038/s41589-020-0554-5" @default.
- W3027782010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32451508" @default.
- W3027782010 hasPublicationYear "2020" @default.
- W3027782010 type Work @default.
- W3027782010 sameAs 3027782010 @default.
- W3027782010 citedByCount "14" @default.
- W3027782010 countsByYear W30277820102020 @default.
- W3027782010 countsByYear W30277820102021 @default.
- W3027782010 countsByYear W30277820102022 @default.
- W3027782010 countsByYear W30277820102023 @default.
- W3027782010 crossrefType "journal-article" @default.
- W3027782010 hasAuthorship W3027782010A5007455893 @default.
- W3027782010 hasAuthorship W3027782010A5009590779 @default.
- W3027782010 hasAuthorship W3027782010A5011500405 @default.
- W3027782010 hasAuthorship W3027782010A5012876343 @default.