FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3028324043> ?p ?o ?g. }

• W3028324043 abstract "Leucine-rich repeat kinase 2 (LRRK2) mutations are among the most significant genetic risk factors for developing late onset Parkinson's disease (PD). To understand whether a therapeutic can modulate LRRK2 levels as a potential disease modifying strategy, it is important to have methods in place to measure the protein with high sensitivity and specificity. To date, LRRK2 measurements in cerebrospinal fluid (CSF) have used extracellular vesicle enrichment via differential ultracentrifugation and western blot detection. Our goal was to develop a methodology which could be deployed in a clinical trial, therefore throughput, robustness and sensitivity were critical. To this end, we developed a Stable Isotope Standard Capture by Anti-peptide Antibody (SISCAPA) assay which is capable of detecting LRRK2 from 1 ml of human CSF. The assay uses a commercially available LRRK2 monoclonal antibody (N241A/34) and does not require extracellular vesicle enrichment steps. The assay includes stable isotope peptide addition which allows for absolute quantitation of LRRK2 protein. We determined that the assay performed adequately for CSF measurements and that blood contamination from traumatic lumbar puncture does not pose a serious analytical challenge. We then applied this technique to 106 CSF samples from the MJFF LRRK2 Cohort Consortium which includes healthy controls, sporadic PD patients and LRRK2 mutation carriers with and without PD. Of the 105 samples that had detectable LRRK2 signal, we found that the PD group with the G2019S LRRK2 mutation had significantly higher CSF LRRK2 levels compared to all other groups. We also found that CSF LRRK2 increased with the age of the participant. Taken together, this work represents a step forward in our ability to measure LRRK2 in a challenging matrix like CSF which has implications for current and future LRRK2 therapeutic clinical trials." @default.
• W3028324043 created "2020-05-29" @default.
• W3028324043 creator A5011477209 @default.
• W3028324043 creator A5011997502 @default.
• W3028324043 creator A5037517823 @default.
• W3028324043 creator A5076296435 @default.
• W3028324043 creator A5086992454 @default.
• W3028324043 creator A5052288750 @default.
• W3028324043 date "2020-05-25" @default.
• W3028324043 modified "2023-10-09" @default.
• W3028324043 title "Quantitative Measurements of LRRK2 in Human Cerebrospinal Fluid Demonstrates Increased Levels in G2019S Patients" @default.
• W3028324043 cites W1977391806 @default.
• W3028324043 cites W1982250218 @default.
• W3028324043 cites W1992365739 @default.
• W3028324043 cites W1992483894 @default.
• W3028324043 cites W2025416265 @default.
• W3028324043 cites W2035037428 @default.
• W3028324043 cites W2046500199 @default.
• W3028324043 cites W2076888837 @default.
• W3028324043 cites W2100867992 @default.
• W3028324043 cites W2103454698 @default.
• W3028324043 cites W2161392725 @default.
• W3028324043 cites W2435254117 @default.
• W3028324043 cites W2739317540 @default.
• W3028324043 cites W2769156514 @default.
• W3028324043 cites W2795590254 @default.
• W3028324043 cites W2883053634 @default.
• W3028324043 cites W2891994821 @default.
• W3028324043 cites W2918195345 @default.
• W3028324043 cites W2970816772 @default.
• W3028324043 cites W2986932567 @default.
• W3028324043 cites W2991331562 @default.
• W3028324043 cites W2996583314 @default.
• W3028324043 cites W3001337291 @default.
• W3028324043 cites W3003392730 @default.
• W3028324043 cites W838038550 @default.
• W3028324043 doi "https://doi.org/10.3389/fnins.2020.00526" @default.
• W3028324043 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7262382" @default.
• W3028324043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32523511" @default.
• W3028324043 hasPublicationYear "2020" @default.
• W3028324043 type Work @default.
• W3028324043 sameAs 3028324043 @default.
• W3028324043 citedByCount "18" @default.
• W3028324043 countsByYear W30283240432020 @default.
• W3028324043 countsByYear W30283240432021 @default.
• W3028324043 countsByYear W30283240432022 @default.
• W3028324043 countsByYear W30283240432023 @default.
• W3028324043 crossrefType "journal-article" @default.
• W3028324043 hasAuthorship W3028324043A5011477209 @default.
• W3028324043 hasAuthorship W3028324043A5011997502 @default.
• W3028324043 hasAuthorship W3028324043A5037517823 @default.
• W3028324043 hasAuthorship W3028324043A5052288750 @default.
• W3028324043 hasAuthorship W3028324043A5076296435 @default.
• W3028324043 hasAuthorship W3028324043A5086992454 @default.
• W3028324043 hasBestOaLocation W30283240431 @default.
• W3028324043 hasConcept C104317684 @default.
• W3028324043 hasConcept C126322002 @default.
• W3028324043 hasConcept C153911025 @default.
• W3028324043 hasConcept C184235292 @default.
• W3028324043 hasConcept C2113261 @default.
• W3028324043 hasConcept C2776415932 @default.
• W3028324043 hasConcept C2779134260 @default.
• W3028324043 hasConcept C2779651940 @default.
• W3028324043 hasConcept C2779734285 @default.
• W3028324043 hasConcept C2780427247 @default.
• W3028324043 hasConcept C28406088 @default.
• W3028324043 hasConcept C55493867 @default.
• W3028324043 hasConcept C71924100 @default.
• W3028324043 hasConcept C86803240 @default.
• W3028324043 hasConceptScore W3028324043C104317684 @default.
• W3028324043 hasConceptScore W3028324043C126322002 @default.
• W3028324043 hasConceptScore W3028324043C153911025 @default.
• W3028324043 hasConceptScore W3028324043C184235292 @default.
• W3028324043 hasConceptScore W3028324043C2113261 @default.
• W3028324043 hasConceptScore W3028324043C2776415932 @default.
• W3028324043 hasConceptScore W3028324043C2779134260 @default.
• W3028324043 hasConceptScore W3028324043C2779651940 @default.
• W3028324043 hasConceptScore W3028324043C2779734285 @default.
• W3028324043 hasConceptScore W3028324043C2780427247 @default.
• W3028324043 hasConceptScore W3028324043C28406088 @default.
• W3028324043 hasConceptScore W3028324043C55493867 @default.
• W3028324043 hasConceptScore W3028324043C71924100 @default.
• W3028324043 hasConceptScore W3028324043C86803240 @default.
• W3028324043 hasLocation W30283240431 @default.
• W3028324043 hasLocation W30283240432 @default.
• W3028324043 hasLocation W30283240433 @default.
• W3028324043 hasLocation W30283240434 @default.
• W3028324043 hasOpenAccess W3028324043 @default.
• W3028324043 hasPrimaryLocation W30283240431 @default.
• W3028324043 hasRelatedWork W181943644 @default.
• W3028324043 hasRelatedWork W1999065406 @default.
• W3028324043 hasRelatedWork W2006195300 @default.
• W3028324043 hasRelatedWork W2034283347 @default.
• W3028324043 hasRelatedWork W2056093558 @default.
• W3028324043 hasRelatedWork W2074474806 @default.
• W3028324043 hasRelatedWork W2166097455 @default.
• W3028324043 hasRelatedWork W2326580452 @default.
• W3028324043 hasRelatedWork W2365771817 @default.
• W3028324043 hasRelatedWork W2419114085 @default.
• W3028324043 hasVolume "14" @default.

• next