FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3028390463> ?p ?o ?g. }

• W3028390463 endingPage "461194" @default.
• W3028390463 startingPage "461194" @default.
• W3028390463 abstract "Quantitative analysis of glycans released from glycoproteins using high-performance liquid chromatography (HPLC) requires fluorescent tag labeling to enhance sensitivity and selectivity. However, the methods required to remove large amounts of excess labeling reagents from the reaction mixture are time-consuming. Furthermore, these methods, including solvent extraction and solid phase extraction (SPE), often impair quantitative analysis. Here, we developed an online sample cleanup procedure for HPLC analysis of 2-aminopyridine (AP)-labeled glycans using a six-port/two-way valve and two small columns: one packed with a strong cation exchange resin (SCX) and the other comprising ODS silica gel. AP-labeled glycans delivered from an injection port were separated from excess AP by passing through an SCX column (4.6 mm i.d., 1 cm long) regulated to 40°C. The AP-labeled glycans were trapped on an ODS column (4.6 mm i.d., 1 cm long) to further separate them from inorganic contaminants. By changing the valve position after 2 min to connect the ODS column to an analysis column, AP-labeled glycans trapped in the ODS column were eluted with an acetonitrile-containing eluent followed by hydrophilic interaction liquid chromatography (HILIC) separation on an amide column or reversed-phase mode separation on a C30 column. This method was successfully used to analyze N-linked glycans released from several glycoprotein samples." @default.
• W3028390463 created "2020-05-29" @default.
• W3028390463 creator A5006641864 @default.
• W3028390463 creator A5009603810 @default.
• W3028390463 creator A5010369760 @default.
• W3028390463 creator A5023381496 @default.
• W3028390463 creator A5086287691 @default.
• W3028390463 creator A5086472574 @default.
• W3028390463 creator A5088662094 @default.
• W3028390463 date "2020-08-01" @default.
• W3028390463 modified "2023-10-17" @default.
• W3028390463 title "Analysis of 2-aminopyridine labeled glycans by dual-mode online solid phase extraction for hydrophilic interaction and reversed-phase liquid chromatography" @default.
• W3028390463 cites W1773393454 @default.
• W3028390463 cites W1787505593 @default.
• W3028390463 cites W1819238680 @default.
• W3028390463 cites W1885817024 @default.
• W3028390463 cites W1907521792 @default.
• W3028390463 cites W1987638542 @default.
• W3028390463 cites W1992857857 @default.
• W3028390463 cites W2004125187 @default.
• W3028390463 cites W2005410518 @default.
• W3028390463 cites W2007312478 @default.
• W3028390463 cites W2007395134 @default.
• W3028390463 cites W2007779878 @default.
• W3028390463 cites W2009874301 @default.
• W3028390463 cites W2013172079 @default.
• W3028390463 cites W2015900004 @default.
• W3028390463 cites W2021303319 @default.
• W3028390463 cites W2023509065 @default.
• W3028390463 cites W2033319529 @default.
• W3028390463 cites W2033726458 @default.
• W3028390463 cites W2038873242 @default.
• W3028390463 cites W2046578718 @default.
• W3028390463 cites W2059347528 @default.
• W3028390463 cites W2068060920 @default.
• W3028390463 cites W2068922409 @default.
• W3028390463 cites W2074589683 @default.
• W3028390463 cites W2078301871 @default.
• W3028390463 cites W2089806857 @default.
• W3028390463 cites W2090690774 @default.
• W3028390463 cites W2103773123 @default.
• W3028390463 cites W2146981871 @default.
• W3028390463 cites W2221488325 @default.
• W3028390463 cites W2512344700 @default.
• W3028390463 cites W2515427808 @default.
• W3028390463 cites W2524986635 @default.
• W3028390463 cites W87884376 @default.
• W3028390463 cites W96640158 @default.
• W3028390463 doi "https://doi.org/10.1016/j.chroma.2020.461194" @default.
• W3028390463 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32709309" @default.
• W3028390463 hasPublicationYear "2020" @default.
• W3028390463 type Work @default.
• W3028390463 sameAs 3028390463 @default.
• W3028390463 citedByCount "5" @default.
• W3028390463 countsByYear W30283904632021 @default.
• W3028390463 countsByYear W30283904632023 @default.
• W3028390463 crossrefType "journal-article" @default.
• W3028390463 hasAuthorship W3028390463A5006641864 @default.
• W3028390463 hasAuthorship W3028390463A5009603810 @default.
• W3028390463 hasAuthorship W3028390463A5010369760 @default.
• W3028390463 hasAuthorship W3028390463A5023381496 @default.
• W3028390463 hasAuthorship W3028390463A5086287691 @default.
• W3028390463 hasAuthorship W3028390463A5086472574 @default.
• W3028390463 hasAuthorship W3028390463A5088662094 @default.
• W3028390463 hasBestOaLocation W30283904631 @default.
• W3028390463 hasConcept C108625454 @default.
• W3028390463 hasConcept C116817701 @default.
• W3028390463 hasConcept C179998833 @default.
• W3028390463 hasConcept C185592680 @default.
• W3028390463 hasConcept C206212055 @default.
• W3028390463 hasConcept C2777463227 @default.
• W3028390463 hasConcept C43617362 @default.
• W3028390463 hasConcept C45188465 @default.
• W3028390463 hasConcept C4725764 @default.
• W3028390463 hasConcept C51724486 @default.
• W3028390463 hasConcept C55493867 @default.
• W3028390463 hasConcept C73826308 @default.
• W3028390463 hasConcept C81426234 @default.
• W3028390463 hasConcept C95986675 @default.
• W3028390463 hasConceptScore W3028390463C108625454 @default.
• W3028390463 hasConceptScore W3028390463C116817701 @default.
• W3028390463 hasConceptScore W3028390463C179998833 @default.
• W3028390463 hasConceptScore W3028390463C185592680 @default.
• W3028390463 hasConceptScore W3028390463C206212055 @default.
• W3028390463 hasConceptScore W3028390463C2777463227 @default.
• W3028390463 hasConceptScore W3028390463C43617362 @default.
• W3028390463 hasConceptScore W3028390463C45188465 @default.
• W3028390463 hasConceptScore W3028390463C4725764 @default.
• W3028390463 hasConceptScore W3028390463C51724486 @default.
• W3028390463 hasConceptScore W3028390463C55493867 @default.
• W3028390463 hasConceptScore W3028390463C73826308 @default.
• W3028390463 hasConceptScore W3028390463C81426234 @default.
• W3028390463 hasConceptScore W3028390463C95986675 @default.
• W3028390463 hasFunder F4320320912 @default.
• W3028390463 hasFunder F4320334764 @default.
• W3028390463 hasLocation W30283904631 @default.
• W3028390463 hasLocation W30283904632 @default.
• W3028390463 hasOpenAccess W3028390463 @default.

• next