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• W3028441834 endingPage "139527" @default.
• W3028441834 startingPage "139527" @default.
• W3028441834 abstract "Today's benthic offshore biological monitoring of oil & gas (O&G) activities relies on macrofauna taxa enumeration. For the future, analysis of DNA isolated directly from sediments holds great potential for multi-trophic biodiversity surveys and the monitoring of a larger spectrum of benthic taxa, including micro-fauna. Here, we evaluate more specifically the potential of microfauna-specific gene quantification in relation to both petroleum-related discharge compounds and other seafloor environmental properties. We carried out this evaluation using sediment samples collected at drilling Region III on the Norwegian continental shelf where DNA metabarcoding of eukaryotic diversity was already performed. Generally, the quantification of microfauna indicator taxa related well to the gradient of contamination on the seafloor. Contrary to eukaryotic Euplotida, metabarcoding data and qPCR numbers for indicative prokaryotic taxa showed the same relationship to offshore contaminants (both showed positive relationship). We found absolute numbers of SSU rRNA gene copies of (1) Dinophyceae, Bacillariophyceae and Alcanivorax were correlated with the level of petroleum-related compounds but not with other environmental variables, (2) bacteria closely related to Shewanella were correlated with the concentration of Ba, PAH, as well to percent of gravel, (3) Desulfobacteriales correlated with petroleum-related contaminants, but as well with percent of gravel and grain size. Findings from our study suggest that biomonitoring surveys of O&G activities on benthos could benefit from quantification of specific micro-fauna indicators that is simpler and faster than the methods currently used for impact assessment of benthos." @default.
• W3028441834 created "2020-05-29" @default.
• W3028441834 creator A5012988770 @default.
• W3028441834 creator A5014955139 @default.
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• W3028441834 date "2020-09-01" @default.
• W3028441834 modified "2023-10-03" @default.
• W3028441834 title "qPCR-based assessment of microfaunal indicators of oil for monitoring benthos around oil and gas platforms" @default.
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• W3028441834 doi "https://doi.org/10.1016/j.scitotenv.2020.139527" @default.
• W3028441834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32497879" @default.
• W3028441834 hasPublicationYear "2020" @default.
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