Matches in SemOpenAlex for { <https://semopenalex.org/work/W3028618972> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W3028618972 endingPage "1168" @default.
- W3028618972 startingPage "1165" @default.
- W3028618972 abstract "Objective To study the relationship between heparanase and cation-independ mannose 6-phosphate receptor (CD222) expression in bladder cancer 5637 cell line.And find out the effect of cell invasiveness by their combination.Methods Immunofluorescence assay were used to detect the position fixing and interaction of heparanase and CD222 in bladder cancer 5637 cell line.The CD222 mRNA-targeted small interfering RNA (siRNA) were designed and constructed.5637 cells were cultured and transfected with siRNA-CD222.The expression of CD222 mRNA and protein were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting.After the recombinant human heparanase were given.Western blotting were performed to detect the expression of CD222 and p-Akt in 5637 cell line before and after tansfection.The invasiveness of above cells were determined by matrigel invasion assays.Results Heparanase and CD222 staining on the cell cytoplasm.Heparanase binds CD222 and cells treated with recombinant human heparanase enhance the binding.Inhibition of CD222 expression in 5637 by siRNA could decrease invasion capacity and the level of Akt phosphorylation which was induced by recombinant human heparanase.Conclusion Heparanase binding to CD222 were localized to the cell cytoplasm and heparanase facilitates bladder cancer cell invasiveness by binding to CD222 and increasing Akt phosphorylation.Key words: Bladder cancer; Hparanase; Cation-independ mannose 6-phosphate receptor; Small interfering RNA; Invasiveness" @default.
- W3028618972 created "2020-06-05" @default.
- W3028618972 creator A5021989628 @default.
- W3028618972 creator A5036479214 @default.
- W3028618972 creator A5041025808 @default.
- W3028618972 creator A5052356366 @default.
- W3028618972 creator A5069316851 @default.
- W3028618972 creator A5089000322 @default.
- W3028618972 date "2014-06-08" @default.
- W3028618972 modified "2023-09-23" @default.
- W3028618972 title "The effect of bladder cancer cell 5637 invasiveness by heparanase through the cation-independ mannose 6-phosphate receptor pathway" @default.
- W3028618972 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2014.06.003" @default.
- W3028618972 hasPublicationYear "2014" @default.
- W3028618972 type Work @default.
- W3028618972 sameAs 3028618972 @default.
- W3028618972 citedByCount "0" @default.
- W3028618972 crossrefType "journal-article" @default.
- W3028618972 hasAuthorship W3028618972A5021989628 @default.
- W3028618972 hasAuthorship W3028618972A5036479214 @default.
- W3028618972 hasAuthorship W3028618972A5041025808 @default.
- W3028618972 hasAuthorship W3028618972A5052356366 @default.
- W3028618972 hasAuthorship W3028618972A5069316851 @default.
- W3028618972 hasAuthorship W3028618972A5089000322 @default.
- W3028618972 hasConcept C104317684 @default.
- W3028618972 hasConcept C11960822 @default.
- W3028618972 hasConcept C1491633281 @default.
- W3028618972 hasConcept C153911025 @default.
- W3028618972 hasConcept C185592680 @default.
- W3028618972 hasConcept C22615655 @default.
- W3028618972 hasConcept C2777478136 @default.
- W3028618972 hasConcept C2778041096 @default.
- W3028618972 hasConcept C502942594 @default.
- W3028618972 hasConcept C54009773 @default.
- W3028618972 hasConcept C54355233 @default.
- W3028618972 hasConcept C55493867 @default.
- W3028618972 hasConcept C75217442 @default.
- W3028618972 hasConcept C81885089 @default.
- W3028618972 hasConcept C85265743 @default.
- W3028618972 hasConcept C86803240 @default.
- W3028618972 hasConcept C95444343 @default.
- W3028618972 hasConceptScore W3028618972C104317684 @default.
- W3028618972 hasConceptScore W3028618972C11960822 @default.
- W3028618972 hasConceptScore W3028618972C1491633281 @default.
- W3028618972 hasConceptScore W3028618972C153911025 @default.
- W3028618972 hasConceptScore W3028618972C185592680 @default.
- W3028618972 hasConceptScore W3028618972C22615655 @default.
- W3028618972 hasConceptScore W3028618972C2777478136 @default.
- W3028618972 hasConceptScore W3028618972C2778041096 @default.
- W3028618972 hasConceptScore W3028618972C502942594 @default.
- W3028618972 hasConceptScore W3028618972C54009773 @default.
- W3028618972 hasConceptScore W3028618972C54355233 @default.
- W3028618972 hasConceptScore W3028618972C55493867 @default.
- W3028618972 hasConceptScore W3028618972C75217442 @default.
- W3028618972 hasConceptScore W3028618972C81885089 @default.
- W3028618972 hasConceptScore W3028618972C85265743 @default.
- W3028618972 hasConceptScore W3028618972C86803240 @default.
- W3028618972 hasConceptScore W3028618972C95444343 @default.
- W3028618972 hasIssue "06" @default.
- W3028618972 hasLocation W30286189721 @default.
- W3028618972 hasOpenAccess W3028618972 @default.
- W3028618972 hasPrimaryLocation W30286189721 @default.
- W3028618972 hasRelatedWork W2002168854 @default.
- W3028618972 hasRelatedWork W2167648889 @default.
- W3028618972 hasRelatedWork W2355326512 @default.
- W3028618972 hasRelatedWork W2356243515 @default.
- W3028618972 hasRelatedWork W2362070413 @default.
- W3028618972 hasRelatedWork W2369674717 @default.
- W3028618972 hasRelatedWork W2379976481 @default.
- W3028618972 hasRelatedWork W2389301143 @default.
- W3028618972 hasRelatedWork W2389316080 @default.
- W3028618972 hasRelatedWork W2392896817 @default.
- W3028618972 hasRelatedWork W2393630770 @default.
- W3028618972 hasRelatedWork W2433150557 @default.
- W3028618972 hasRelatedWork W2751459212 @default.
- W3028618972 hasRelatedWork W2767595741 @default.
- W3028618972 hasRelatedWork W3028688407 @default.
- W3028618972 hasRelatedWork W3029182286 @default.
- W3028618972 hasRelatedWork W3030178654 @default.
- W3028618972 hasRelatedWork W3030918658 @default.
- W3028618972 hasRelatedWork W3032776547 @default.
- W3028618972 hasRelatedWork W91061769 @default.
- W3028618972 hasVolume "31" @default.
- W3028618972 isParatext "false" @default.
- W3028618972 isRetracted "false" @default.
- W3028618972 magId "3028618972" @default.
- W3028618972 workType "article" @default.