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- W3028709296 abstract "Objective To investigate the impact of laparoscopic gastrointestinal surgery on serum protein expression in patients with type 2 diabetes mellitus(T2DM).Methods Twelve patients with T2DM received gastrointestinal surgery at Changhai Hospital of the Second Medical University from June 2008 to September 2010.Their serum samples were collected at different time points(before surgery,1 week and 1 month after surgery).Total proteins were seperated by two-dimensional(2D)gel electrophoresis.The differentially expressed proteins were analyzed by mass spectrometry and bioinformatics.Results Protein extracts of the serum samples were separated on 2D gels successfully.Twenty differentially expressed proteins in the serum after surgery were screened out.Eight proteins were successfully identified,in which the expression of 5 proteins(Rho GDP-dissociation inhibitor 1,Prohibitin,Alpha-1-anfitrypsin precursor,Serotransferrin precursor and Fibrinogen gamma chain precursor)was increased after operation,and the expression of 3 proteins(MAP3K12-binding inhibitory protein 1,Coronin-1A and Isovalery1-COA dehydrogenase) was decreased.Conclusions The expression of 20 proteins have been changed significantly in serum samples after laparoscopic gastrointestinal surgery in patients with T2DM,and 8 proteins were successfully identified.Key words: Diabetes mellitus,type 2; Gastrointestinal surgery; Laparoscopy; Proteome; Twodimensional gel electrophoresis" @default.
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- W3028709296 date "2011-06-20" @default.
- W3028709296 modified "2023-09-27" @default.
- W3028709296 title "Serum proteomic analysis after laparoscopic gastrointestinal surgery for the treatment of type 2 diabetes mellitus" @default.
- W3028709296 doi "https://doi.org/10.3760/cma.j.issn.1673-9752.2011.03.018" @default.
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