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- W3028752457 abstract "目的 建立ELISA法检测疫苗中残留卵清蛋白的含量.方法 将进口纯化兔抗卵清蛋白抗体作为包被抗体,辣根过氧化物酶标记兔抗卵清蛋白抗体作为酶标抗体,以系列含量的卵清蛋白溶液为标准,采用ELISA双抗体夹心法,对供试品中残留卵清蛋白进行定量测定.结果 最佳线性范围为1.25~20 ng/ml,相关系数r≥0.99;定量限度为1.25 ng/ml;准确性试验回收率为89.2%~103.6%;精密性试验内和试验间变异系数分别为5.6%~6.7%和4.2%~9.4%.与马血清、羊血清、人血清白蛋白、牛血清白蛋白、流行性感冒病毒裂解疫苗基质液及其他无残留卵清蛋白的疫苗均无交叉反应.结论 本方法特异性强、灵敏度高,准确性、重复性和稳定性好,可用于疫苗中残留卵清蛋白的定量检测。" @default.
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- W3028752457 date "2007-04-10" @default.
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- W3028752457 title "Quantitative determination of residual ovalbumin in vaccine by ELISA" @default.
- W3028752457 doi "https://doi.org/10.3760/cma.j.issn.1673-4211.2007.02.002" @default.
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