FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3028778833> ?p ?o ?g. }

• W3028778833 endingPage "109642" @default.
• W3028778833 startingPage "109642" @default.
• W3028778833 abstract "In this study, we established a novel polymerase chain reaction-hybridization chain reaction (PCR-HCR) dual signal amplification assay for sensitive detection of Escherichia coli O157:H7 in milk. In this assay, a blocker forward primer and a biotin reverse primer based on E. coli O157:H7 specific fliCh7 gene were specifically designed for PCR amplification resulting in a single-double stranded DNA with biotin (ss-dsDNA~biotin). These PCR products, ss-dsDNA~biotin, were captured by magnetic beads (MBs) decorated with streptavidin (SA) forming the ss-dsDNA~biotin+SA~MBs. The ssDNA on the PCR products opened the hairpin probe (H1 and H2) to trigger the HCR for secondary signal amplification. This is the first to use HCR to directly amplify PCR products through a novel blocker primer. With the help of two nucleic acid dual signal amplification techniques, PCR-HCR analysis can effectively improve the detection sensitivity and the limit of detection is 7.2 × 101 CFU/mL. In addition, this assay has been successfully used to detection target bacteria in skim milk spiked samples." @default.
• W3028778833 created "2020-06-05" @default.
• W3028778833 creator A5013689462 @default.
• W3028778833 creator A5027371837 @default.
• W3028778833 creator A5038757746 @default.
• W3028778833 creator A5078793883 @default.
• W3028778833 creator A5080243083 @default.
• W3028778833 creator A5084407792 @default.
• W3028778833 date "2020-08-01" @default.
• W3028778833 modified "2023-10-12" @default.
• W3028778833 title "The PCR-HCR dual signal amplification strategy for ultrasensitive detection of Escherichia coli O157:H7 in milk" @default.
• W3028778833 cites W1932713539 @default.
• W3028778833 cites W2019743688 @default.
• W3028778833 cites W2051632571 @default.
• W3028778833 cites W2075103182 @default.
• W3028778833 cites W2082241802 @default.
• W3028778833 cites W2087021336 @default.
• W3028778833 cites W2090645955 @default.
• W3028778833 cites W2106010955 @default.
• W3028778833 cites W2115313390 @default.
• W3028778833 cites W2148629428 @default.
• W3028778833 cites W2156896172 @default.
• W3028778833 cites W2167497676 @default.
• W3028778833 cites W2318673848 @default.
• W3028778833 cites W2409428719 @default.
• W3028778833 cites W2478255373 @default.
• W3028778833 cites W2489349695 @default.
• W3028778833 cites W2506271165 @default.
• W3028778833 cites W2590038892 @default.
• W3028778833 cites W2621451166 @default.
• W3028778833 cites W2737217819 @default.
• W3028778833 cites W2752239237 @default.
• W3028778833 cites W2753521484 @default.
• W3028778833 cites W2767974482 @default.
• W3028778833 cites W2769449271 @default.
• W3028778833 cites W2809456967 @default.
• W3028778833 cites W2892969641 @default.
• W3028778833 cites W2893933029 @default.
• W3028778833 cites W2897072773 @default.
• W3028778833 cites W2915287394 @default.
• W3028778833 cites W2921161382 @default.
• W3028778833 cites W806431811 @default.
• W3028778833 doi "https://doi.org/10.1016/j.lwt.2020.109642" @default.
• W3028778833 hasPublicationYear "2020" @default.
• W3028778833 type Work @default.
• W3028778833 sameAs 3028778833 @default.
• W3028778833 citedByCount "9" @default.
• W3028778833 countsByYear W30287788332020 @default.
• W3028778833 countsByYear W30287788332021 @default.
• W3028778833 countsByYear W30287788332022 @default.
• W3028778833 countsByYear W30287788332023 @default.
• W3028778833 crossrefType "journal-article" @default.
• W3028778833 hasAuthorship W3028778833A5013689462 @default.
• W3028778833 hasAuthorship W3028778833A5027371837 @default.
• W3028778833 hasAuthorship W3028778833A5038757746 @default.
• W3028778833 hasAuthorship W3028778833A5078793883 @default.
• W3028778833 hasAuthorship W3028778833A5080243083 @default.
• W3028778833 hasAuthorship W3028778833A5084407792 @default.
• W3028778833 hasConcept C104317684 @default.
• W3028778833 hasConcept C119128265 @default.
• W3028778833 hasConcept C153911025 @default.
• W3028778833 hasConcept C178790620 @default.
• W3028778833 hasConcept C185592680 @default.
• W3028778833 hasConcept C24107716 @default.
• W3028778833 hasConcept C2776190903 @default.
• W3028778833 hasConcept C2777563447 @default.
• W3028778833 hasConcept C2778204606 @default.
• W3028778833 hasConcept C43617362 @default.
• W3028778833 hasConcept C48023723 @default.
• W3028778833 hasConcept C49105822 @default.
• W3028778833 hasConcept C52001828 @default.
• W3028778833 hasConcept C547475151 @default.
• W3028778833 hasConcept C55493867 @default.
• W3028778833 hasConcept C86803240 @default.
• W3028778833 hasConcept C90583042 @default.
• W3028778833 hasConceptScore W3028778833C104317684 @default.
• W3028778833 hasConceptScore W3028778833C119128265 @default.
• W3028778833 hasConceptScore W3028778833C153911025 @default.
• W3028778833 hasConceptScore W3028778833C178790620 @default.
• W3028778833 hasConceptScore W3028778833C185592680 @default.
• W3028778833 hasConceptScore W3028778833C24107716 @default.
• W3028778833 hasConceptScore W3028778833C2776190903 @default.
• W3028778833 hasConceptScore W3028778833C2777563447 @default.
• W3028778833 hasConceptScore W3028778833C2778204606 @default.
• W3028778833 hasConceptScore W3028778833C43617362 @default.
• W3028778833 hasConceptScore W3028778833C48023723 @default.
• W3028778833 hasConceptScore W3028778833C49105822 @default.
• W3028778833 hasConceptScore W3028778833C52001828 @default.
• W3028778833 hasConceptScore W3028778833C547475151 @default.
• W3028778833 hasConceptScore W3028778833C55493867 @default.
• W3028778833 hasConceptScore W3028778833C86803240 @default.
• W3028778833 hasConceptScore W3028778833C90583042 @default.
• W3028778833 hasFunder F4320322792 @default.
• W3028778833 hasFunder F4320326881 @default.
• W3028778833 hasFunder F4320335777 @default.
• W3028778833 hasLocation W30287788331 @default.
• W3028778833 hasOpenAccess W3028778833 @default.
• W3028778833 hasPrimaryLocation W30287788331 @default.

• next