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- W3028785361 abstract "ObjectiveTo investigate the effect of TNF-α and IL-1β on the expression of human pancreatic cancer PaTu8988 NF-κB and Hedgehog (HH) signaling pathways members Gli1, Shh, SMO, SuFu.MethodsPancreatic cancer cell line PaTu8988 was treated with TNF-α and IL-1β, respectively, for 48 h, and cells without treatment were control group. Then the protein and mRNA expression of NF-κB and Shh, SMO, Gli1, SuFu was determined by RT-PCR and Western blot; apoptosis of cells were tested by flow cytometry.ResultsThe expressions of NF-κB mRNA in PaTu8988 cells in TNF-α group, IL-1β group, control group were 9.92±0.78, 7.74±0.32, 1.01±0.08; and the expressions of Gli1 mRNA were 7.25±0.45, 5.74±0.33, 1.00±0.06; and the expressions of Shh mRNA were 3.60±0.36, 4.33±0.45, 1.00±0.04; and the expressions of SMO mRNA were 1.03±0.15, 1.07±0.16, 1.01±0.06; and the expressions of SuFu mRNA were 0.88±0.14, 0.96±0.13, 1.01±0.05. The expressions of NF-κB, Gli1, Shh mRNA in TNF-α group, IL-1β group were significantly higher than that in control group, and the difference between the two groups was statistically significant (P<0.05). The expressions of corresponding proteins were consistent with the expressions of mRNA. The apoptosis rates in TNF-α group, IL-1β group, control group were (17.40±2.87)%, (11.05±1.34)%, (49.90±2.96)%, and the apoptosis rates in TNF-α group, IL-1β group were significantly lower than that in control group, and the difference between the two groups was statistically significant (P<0.01).ConclusionsTNF-α and IL-1β can activate the expression of NF-κB and Shh, Gli1 in human pancreatic cancer PaTu898, and decrease apoptosis.Key words: Pancreatic neoplasms; Hedgehog proteins; Signal transduction; Inflammation; NF-kappa B" @default.
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- W3028785361 date "2015-02-20" @default.
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- W3028785361 title "Pro-inflammatory cytokines inhibit apoptosis of pancreatic ductal adenocarcinoma cells via both NF-κB and Hedgehog signaling pathways" @default.
- W3028785361 doi "https://doi.org/10.3760/cma.j.issn.1674-1935.2015.01.005" @default.
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