Matches in SemOpenAlex for { <https://semopenalex.org/work/W3028932094> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W3028932094 abstract "Detection of the protein antigens in immunoblots prepared with immunoprecipitated protein antigens can be affected by the presence of high amounts of the immunoprecipitating antibodies. When it is not possible to use the immunoprecipitating antibodies and the primary antibodies raised in different species, this protocol provides a convenient and inexpensive alternative to achieve optimal detection of immunoprecipitated protein antigens. In this protocol, a nitrocellulose or polyvinylidene fluoride membrane containing immunoprecipitated protein samples is rinsed with ultrapure H2O after the transfer of proteins and detection of the total proteins using Ponceau S dye (optional). Blocking solution is applied to the membrane, and the membrane is incubated and then rinsed off (optional) before addition of the primary antibody labeled with biotin. After washing, the membrane is incubated with enzyme- or fluorochrome-labeled avidin for detection." @default.
- W3028932094 created "2020-06-05" @default.
- W3028932094 creator A5073477931 @default.
- W3028932094 date "2020-06-01" @default.
- W3028932094 modified "2023-10-14" @default.
- W3028932094 title "Blocking of Immunoblots and Incubation with Antibodies: Procedure for Immunoblots Prepared with Immunoprecipitated Protein Antigens" @default.
- W3028932094 cites W2036623463 @default.
- W3028932094 cites W2894564965 @default.
- W3028932094 cites W2966801458 @default.
- W3028932094 cites W3010622974 @default.
- W3028932094 doi "https://doi.org/10.1101/pdb.prot098475" @default.
- W3028932094 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32482899" @default.
- W3028932094 hasPublicationYear "2020" @default.
- W3028932094 type Work @default.
- W3028932094 sameAs 3028932094 @default.
- W3028932094 citedByCount "1" @default.
- W3028932094 countsByYear W30289320942020 @default.
- W3028932094 crossrefType "journal-article" @default.
- W3028932094 hasAuthorship W3028932094A5073477931 @default.
- W3028932094 hasConcept C104317684 @default.
- W3028932094 hasConcept C144647389 @default.
- W3028932094 hasConcept C147483822 @default.
- W3028932094 hasConcept C153911025 @default.
- W3028932094 hasConcept C159654299 @default.
- W3028932094 hasConcept C171250308 @default.
- W3028932094 hasConcept C185592680 @default.
- W3028932094 hasConcept C192562407 @default.
- W3028932094 hasConcept C203014093 @default.
- W3028932094 hasConcept C21269302 @default.
- W3028932094 hasConcept C2778954975 @default.
- W3028932094 hasConcept C3019566124 @default.
- W3028932094 hasConcept C41625074 @default.
- W3028932094 hasConcept C43617362 @default.
- W3028932094 hasConcept C55493867 @default.
- W3028932094 hasConcept C7051213 @default.
- W3028932094 hasConcept C71829478 @default.
- W3028932094 hasConcept C86803240 @default.
- W3028932094 hasConceptScore W3028932094C104317684 @default.
- W3028932094 hasConceptScore W3028932094C144647389 @default.
- W3028932094 hasConceptScore W3028932094C147483822 @default.
- W3028932094 hasConceptScore W3028932094C153911025 @default.
- W3028932094 hasConceptScore W3028932094C159654299 @default.
- W3028932094 hasConceptScore W3028932094C171250308 @default.
- W3028932094 hasConceptScore W3028932094C185592680 @default.
- W3028932094 hasConceptScore W3028932094C192562407 @default.
- W3028932094 hasConceptScore W3028932094C203014093 @default.
- W3028932094 hasConceptScore W3028932094C21269302 @default.
- W3028932094 hasConceptScore W3028932094C2778954975 @default.
- W3028932094 hasConceptScore W3028932094C3019566124 @default.
- W3028932094 hasConceptScore W3028932094C41625074 @default.
- W3028932094 hasConceptScore W3028932094C43617362 @default.
- W3028932094 hasConceptScore W3028932094C55493867 @default.
- W3028932094 hasConceptScore W3028932094C7051213 @default.
- W3028932094 hasConceptScore W3028932094C71829478 @default.
- W3028932094 hasConceptScore W3028932094C86803240 @default.
- W3028932094 hasLocation W30289320941 @default.
- W3028932094 hasOpenAccess W3028932094 @default.
- W3028932094 hasPrimaryLocation W30289320941 @default.
- W3028932094 hasRelatedWork W13352498 @default.
- W3028932094 hasRelatedWork W1405714 @default.
- W3028932094 hasRelatedWork W16535465 @default.
- W3028932094 hasRelatedWork W19822233 @default.
- W3028932094 hasRelatedWork W27733117 @default.
- W3028932094 hasRelatedWork W27802181 @default.
- W3028932094 hasRelatedWork W32433696 @default.
- W3028932094 hasRelatedWork W33782608 @default.
- W3028932094 hasRelatedWork W5214742 @default.
- W3028932094 hasRelatedWork W9245075 @default.
- W3028932094 isParatext "false" @default.
- W3028932094 isRetracted "false" @default.
- W3028932094 magId "3028932094" @default.
- W3028932094 workType "article" @default.