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- W3028937040 abstract "Objective To transduct with human telomerase reverse transcriptase(hTERT) to Schwann cells via electransfection technique to prolong the life span and enhance the proliferative capacity of Schwann cells.Methods The hTERT RNA was derived from esophagus cancer tissue and pcDNA3.1-hTERT vector was built.With electransfection technique, we tmnsfected vector into ADSCs.The PCR,TRAP-PCR, and PI-annexin V were tested to prove the expression of hTERT in ADSCs.Results After transduction the hTERT RNA in Schwann ceils, TRAP-PCR test, PI-annexin V dying and S100 dying was positive.Conclusion Telomerase catalytic subunit(hTERT) is transduct into Schwann cells, and it can to enhance the prolifertative capacity.Key words: Telomerase; Schwann cells ; Electransfection" @default.
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- W3028937040 date "2010-06-25" @default.
- W3028937040 modified "2023-09-24" @default.
- W3028937040 title "Electransfection with telomerase catalytic subunit(hTERT) enhances proliferative capacity of Schwann cells" @default.
- W3028937040 doi "https://doi.org/10.3760/cma.j.issn.1001-2036.2010.03.017" @default.
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