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- W3029116495 abstract "Objective To investgate the effect of macrophage tumor vaccine on the regulation of CTL response and Th1/Th2 type cytokines production. Methods The tumor cell injury rate and the level of LDH in culture pressed in spleen cells and released into culture supernatant were detected with Western bolting and ELISA, re-spectively. Results The tumor injury rate and the level of LDH in culture supernatant of macrophage tumor vaccine immunized group were (36. 70 +21.02)% and (0. 29 +0. 15) U/ml, respectively. Both are signifi-cantly higher than that of the group immunized with heat inactivated tumor cells and the group immunized with liquid paraffin induced macrophages (P < 0. 05). After stimulating 72 hours with the freeze thawing tumor cells, IL-10 and IFN-T in culture supernatant of macrophage tumor vaccine immunized group were (7. 97 ±3. 15) pg/ml, (0. 44 ±0. 11) pg/ml, (1.83±0. 85) pg/ml and (9. 16±4. 64) pg/ml, respectively. All of them obvi-ously higher than that of the group of immunized with heat inactivated tumor cells and the group immunized with liquid paraffin induced macrophages(P <0. 05). Condusion Macrophage tumor vaccine could elicit specific Key words: CTL response; Macrophage tumor vaccine; Cytokine" @default.
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- W3029116495 date "2009-03-05" @default.
- W3029116495 modified "2023-09-23" @default.
- W3029116495 title "Observation on the immunoregulation function of the macrophage tumor vaccine" @default.
- W3029116495 doi "https://doi.org/10.3760/cma.j.issn.1673-4394.2009.02.003" @default.
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