Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029122054> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W3029122054 endingPage "839" @default.
- W3029122054 startingPage "836" @default.
- W3029122054 abstract "目的 检测survivin基因在卵巢上皮性癌(卵巢癌)耐药细胞株SKOV3/ADM及其亲本细胞株SKOV3中的表达水平,探讨以survivin基因为靶向的RNA干扰(RNAi)能否有效沉默survivin基因在SKOV3/ADM细胞中的表达,并能否有效逆转SKOV3/ADM细胞对化疗药物的耐药性.方法半定量RT-PCR、免疫荧光法检测survivin mRNA、蛋白在SKOV3/ADM及SKOV3细胞中的表达.以survivin基因为靶向的重组质粒pshRNA-survivin及紫杉醇作用于SKOV3/ADM细胞,将SKOV3/ADM细胞分为4组,即对照组、RNAi组、紫杉醇组、RNAi+紫杉醇组,RT-PCR技术检测各组细胞的survivin mRNA表达水平,吖啶橙/溴乙锭(AO/EB)荧光染色法及末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸标记法(TUNEL)检测各组细胞的凋亡情况.结果 survivin mRNA在SKOV3/ADM、SKOV3细胞中的表达水平分别为99.1%、75.3%,SKOV3/ADM、SKOV3细胞的荧光细胞数分别为(59±5)、(42±3)个,两种细胞间比较,差异均有统计学意义(P<0.05).RNAi后SKOV3/ADM细胞中 mRNA的表达水平由99.1%降低至7.9%.AO/EB荧光染色法、TUNEL法检测各组细胞凋亡数,紫杉醇组为(10.2±1.0)、(9.8±0.8)个,RNAi组为(48.5±4.9)、(49.5±4.3)个,RNAi+紫杉醇组为(71.5±6.8)、(68.3±5.7)个,RNAi+紫杉醇组与RNAi组比较,差异有统计学意义(P<0.05),RNAi+紫杉醇组与紫杉醇组比较,差异也有统计学意义(P<0.05).结论 survivin基因在SKOV3/ADM细胞中的表达显著高于SKOV3细胞.以survivin基因为靶向的RNAi可有效抑制SKOV3/ADM细胞中survivin基因的表达,有效增加了SKOV3/ADM细胞对化疗药物紫杉醇的敏感性,显著上调了SKOV3/ADM细胞的凋亡活性。" @default.
- W3029122054 created "2020-06-05" @default.
- W3029122054 creator A5003673117 @default.
- W3029122054 creator A5012872494 @default.
- W3029122054 creator A5041465477 @default.
- W3029122054 creator A5044285445 @default.
- W3029122054 creator A5080790465 @default.
- W3029122054 date "2005-12-18" @default.
- W3029122054 modified "2023-09-25" @default.
- W3029122054 title "RNA interference silencing expression of survivin gene and reversing drug resistance of ovarian cancer cell line SKOV3/ADM" @default.
- W3029122054 doi "https://doi.org/10.3760/j.issn:0529-567x.2005.12.012" @default.
- W3029122054 hasPublicationYear "2005" @default.
- W3029122054 type Work @default.
- W3029122054 sameAs 3029122054 @default.
- W3029122054 citedByCount "0" @default.
- W3029122054 crossrefType "journal-article" @default.
- W3029122054 hasAuthorship W3029122054A5003673117 @default.
- W3029122054 hasAuthorship W3029122054A5012872494 @default.
- W3029122054 hasAuthorship W3029122054A5041465477 @default.
- W3029122054 hasAuthorship W3029122054A5044285445 @default.
- W3029122054 hasAuthorship W3029122054A5080790465 @default.
- W3029122054 hasConcept C104317684 @default.
- W3029122054 hasConcept C114851261 @default.
- W3029122054 hasConcept C119056186 @default.
- W3029122054 hasConcept C121608353 @default.
- W3029122054 hasConcept C139278185 @default.
- W3029122054 hasConcept C150194340 @default.
- W3029122054 hasConcept C153911025 @default.
- W3029122054 hasConcept C159985019 @default.
- W3029122054 hasConcept C166703698 @default.
- W3029122054 hasConcept C190232843 @default.
- W3029122054 hasConcept C192562407 @default.
- W3029122054 hasConcept C2191507 @default.
- W3029122054 hasConcept C22615655 @default.
- W3029122054 hasConcept C2775975398 @default.
- W3029122054 hasConcept C2780427987 @default.
- W3029122054 hasConcept C2781085045 @default.
- W3029122054 hasConcept C502942594 @default.
- W3029122054 hasConcept C54355233 @default.
- W3029122054 hasConcept C67705224 @default.
- W3029122054 hasConcept C81885089 @default.
- W3029122054 hasConcept C86803240 @default.
- W3029122054 hasConceptScore W3029122054C104317684 @default.
- W3029122054 hasConceptScore W3029122054C114851261 @default.
- W3029122054 hasConceptScore W3029122054C119056186 @default.
- W3029122054 hasConceptScore W3029122054C121608353 @default.
- W3029122054 hasConceptScore W3029122054C139278185 @default.
- W3029122054 hasConceptScore W3029122054C150194340 @default.
- W3029122054 hasConceptScore W3029122054C153911025 @default.
- W3029122054 hasConceptScore W3029122054C159985019 @default.
- W3029122054 hasConceptScore W3029122054C166703698 @default.
- W3029122054 hasConceptScore W3029122054C190232843 @default.
- W3029122054 hasConceptScore W3029122054C192562407 @default.
- W3029122054 hasConceptScore W3029122054C2191507 @default.
- W3029122054 hasConceptScore W3029122054C22615655 @default.
- W3029122054 hasConceptScore W3029122054C2775975398 @default.
- W3029122054 hasConceptScore W3029122054C2780427987 @default.
- W3029122054 hasConceptScore W3029122054C2781085045 @default.
- W3029122054 hasConceptScore W3029122054C502942594 @default.
- W3029122054 hasConceptScore W3029122054C54355233 @default.
- W3029122054 hasConceptScore W3029122054C67705224 @default.
- W3029122054 hasConceptScore W3029122054C81885089 @default.
- W3029122054 hasConceptScore W3029122054C86803240 @default.
- W3029122054 hasIssue "12" @default.
- W3029122054 hasLocation W30291220541 @default.
- W3029122054 hasOpenAccess W3029122054 @default.
- W3029122054 hasPrimaryLocation W30291220541 @default.
- W3029122054 hasRelatedWork W2132937043 @default.
- W3029122054 hasRelatedWork W2244049009 @default.
- W3029122054 hasRelatedWork W2348868603 @default.
- W3029122054 hasRelatedWork W2349512530 @default.
- W3029122054 hasRelatedWork W2350787278 @default.
- W3029122054 hasRelatedWork W2353597317 @default.
- W3029122054 hasRelatedWork W2354184704 @default.
- W3029122054 hasRelatedWork W2356773887 @default.
- W3029122054 hasRelatedWork W2356924086 @default.
- W3029122054 hasRelatedWork W2376302752 @default.
- W3029122054 hasRelatedWork W2381126824 @default.
- W3029122054 hasRelatedWork W2387123281 @default.
- W3029122054 hasRelatedWork W2387420200 @default.
- W3029122054 hasRelatedWork W2388385446 @default.
- W3029122054 hasRelatedWork W2391639669 @default.
- W3029122054 hasRelatedWork W2999167257 @default.
- W3029122054 hasRelatedWork W3029957805 @default.
- W3029122054 hasRelatedWork W3031839577 @default.
- W3029122054 hasRelatedWork W3138138639 @default.
- W3029122054 hasRelatedWork W3149132411 @default.
- W3029122054 hasVolume "40" @default.
- W3029122054 isParatext "false" @default.
- W3029122054 isRetracted "false" @default.
- W3029122054 magId "3029122054" @default.
- W3029122054 workType "article" @default.