Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029123737> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W3029123737 abstract "Erythroxylum cuneatum is a member of a tropical plant family which is considered to be a good source of phenolic compounds. The aims of this study were to determine the in vitro cytotoxicity, cytoprotective activity, genotoxicity and antioxidant enzymes specific activities of the standardized aqueous extract of E. cuneatum. In vitro toxicity of E. cuneatum extract in four cultured cell lines was determined. The IC50 values in the various cell lines were all higher than 100 µg/ml, indicating low cytotoxic effects. The cytoprotective activity was determined by treating four cultured cell lines (leukemia cells, colon cancer cells, liver cancer cells and normal liver cells) with hydrogen peroxide H2O2 (28-158 µM) and determining the effects of E. cuneatum (5-50 µg/ml) in the H2O2 treated cells. The results showed that H2O2 was cytotoxic to cells and the addition of E. cuneatum reduced its cytotoxicity. The cytoprotective activity was also determined by treating cultured cell lines (liver cancer cells and normal liver cells) with menadione (2methyl- l A-naphthoquinone) (12-30 µM) and determining the effects of the extract (5-50 µg/ml) in the menadione-treated cells. Results showed that the extract, was not able to rescue cells from the cytotoxicity effects of menadione. Genotoxicity of the extract in HepG2 and WRL68 cell lines were assessed by single cell gel electrophoresis (comet assay), whereby cells were treated with hydrogen peroxide (50 µM) as genotoxic control and different concentrations of E. cuneatum (0.005-1 mg/ml). A low level of DNA damage was seen at the highest concentration of 1 mg/ml while no DNA damage was seen at the other concentrations. E. cuneatum 50 µg/ml when incubated with normal and cancer liver cells significantly increased in catalase specific activity but did not affect cellular GPx and SOD activities. E. cuneatum standardized aqueous extract was found to be non cytotoxic and non genotoxic, showed protection to HepG2 and HCT116 cells from H2O2- induced toxicity but did alter menadione induced cell death. E. cuneatum (50 µg/ml) elevated catalase specific activity in HepG2 and WRL68 cells. More studies are needed if E. cuneatum is to be developed as a cytoprotectant." @default.
- W3029123737 created "2020-06-05" @default.
- W3029123737 creator A5053167058 @default.
- W3029123737 date "2010-01-01" @default.
- W3029123737 modified "2023-09-24" @default.
- W3029123737 title "Safety and efficacy of erythroxylum cuneatum / Wesam Radhi Kadhum" @default.
- W3029123737 hasPublicationYear "2010" @default.
- W3029123737 type Work @default.
- W3029123737 sameAs 3029123737 @default.
- W3029123737 citedByCount "0" @default.
- W3029123737 crossrefType "dissertation" @default.
- W3029123737 hasAuthorship W3029123737A5053167058 @default.
- W3029123737 hasConcept C109316439 @default.
- W3029123737 hasConcept C121608353 @default.
- W3029123737 hasConcept C143425029 @default.
- W3029123737 hasConcept C153911025 @default.
- W3029123737 hasConcept C154317977 @default.
- W3029123737 hasConcept C178790620 @default.
- W3029123737 hasConcept C181199279 @default.
- W3029123737 hasConcept C181674461 @default.
- W3029123737 hasConcept C185592680 @default.
- W3029123737 hasConcept C202751555 @default.
- W3029123737 hasConcept C2778959862 @default.
- W3029123737 hasConcept C2780358455 @default.
- W3029123737 hasConcept C2781095387 @default.
- W3029123737 hasConcept C29730261 @default.
- W3029123737 hasConcept C533411734 @default.
- W3029123737 hasConcept C54355233 @default.
- W3029123737 hasConcept C552990157 @default.
- W3029123737 hasConcept C55493867 @default.
- W3029123737 hasConcept C81885089 @default.
- W3029123737 hasConcept C86803240 @default.
- W3029123737 hasConcept C96232424 @default.
- W3029123737 hasConcept C98274493 @default.
- W3029123737 hasConceptScore W3029123737C109316439 @default.
- W3029123737 hasConceptScore W3029123737C121608353 @default.
- W3029123737 hasConceptScore W3029123737C143425029 @default.
- W3029123737 hasConceptScore W3029123737C153911025 @default.
- W3029123737 hasConceptScore W3029123737C154317977 @default.
- W3029123737 hasConceptScore W3029123737C178790620 @default.
- W3029123737 hasConceptScore W3029123737C181199279 @default.
- W3029123737 hasConceptScore W3029123737C181674461 @default.
- W3029123737 hasConceptScore W3029123737C185592680 @default.
- W3029123737 hasConceptScore W3029123737C202751555 @default.
- W3029123737 hasConceptScore W3029123737C2778959862 @default.
- W3029123737 hasConceptScore W3029123737C2780358455 @default.
- W3029123737 hasConceptScore W3029123737C2781095387 @default.
- W3029123737 hasConceptScore W3029123737C29730261 @default.
- W3029123737 hasConceptScore W3029123737C533411734 @default.
- W3029123737 hasConceptScore W3029123737C54355233 @default.
- W3029123737 hasConceptScore W3029123737C552990157 @default.
- W3029123737 hasConceptScore W3029123737C55493867 @default.
- W3029123737 hasConceptScore W3029123737C81885089 @default.
- W3029123737 hasConceptScore W3029123737C86803240 @default.
- W3029123737 hasConceptScore W3029123737C96232424 @default.
- W3029123737 hasConceptScore W3029123737C98274493 @default.
- W3029123737 hasLocation W30291237371 @default.
- W3029123737 hasOpenAccess W3029123737 @default.
- W3029123737 hasPrimaryLocation W30291237371 @default.
- W3029123737 hasRelatedWork W1970152780 @default.
- W3029123737 hasRelatedWork W2000116528 @default.
- W3029123737 hasRelatedWork W2024955437 @default.
- W3029123737 hasRelatedWork W2076647380 @default.
- W3029123737 hasRelatedWork W2142045044 @default.
- W3029123737 hasRelatedWork W2181841999 @default.
- W3029123737 hasRelatedWork W2201258486 @default.
- W3029123737 hasRelatedWork W2332330445 @default.
- W3029123737 hasRelatedWork W2620543416 @default.
- W3029123737 hasRelatedWork W2808386535 @default.
- W3029123737 hasRelatedWork W2884514928 @default.
- W3029123737 hasRelatedWork W3001230575 @default.
- W3029123737 hasRelatedWork W3042730392 @default.
- W3029123737 hasRelatedWork W3043052449 @default.
- W3029123737 hasRelatedWork W3072737713 @default.
- W3029123737 hasRelatedWork W3160940999 @default.
- W3029123737 hasRelatedWork W3181424366 @default.
- W3029123737 hasRelatedWork W2184694547 @default.
- W3029123737 hasRelatedWork W2187079492 @default.
- W3029123737 hasRelatedWork W3016207635 @default.
- W3029123737 isParatext "false" @default.
- W3029123737 isRetracted "false" @default.
- W3029123737 magId "3029123737" @default.
- W3029123737 workType "dissertation" @default.