Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029125761> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3029125761 endingPage "733" @default.
- W3029125761 startingPage "728" @default.
- W3029125761 abstract "ObjectiveTo study the role of Cx43 in X-ray induced apoptosis of HUVEC cells and its mechanism.MethodsFlow cytometry was used to detect the apoptosis of HUVEC cells at 48-96 h after 10 Gy X-ray irradiation and at 72 h after irradiation of different doses. Western blot was used to detect the protein expressions of Cx43 and cleaved caspase-3 in HUVEC cells at 72 h after 0, 5, 10 and 20 Gy irradiation. Small interfering RNA was transfected into HUVEC cells to silence Cx43 expression, the Cx43 bearing plasmid was transfected into cells to overexpress Cx43. The effect of Cx43 knockdown or overexpression on apoptosis induction and cleaved caspase-3 protein expression were detected by flow cytometry and Western blot, respectively.ResultsThe apoptosis of HUVEC increased significantly from 48 h to 96 h after X-ray irradiation and in a dose-dependent manner at 72 h after irradiation. The expression of Cx43 protein was negatively correlated with the dose but the expression of cleaved caspase-3 was positively correlated with the dose in the range of 0-20 Gy. After Cx43 silencing, the proportion of early apoptosis and apoptosis combined with dead cells were significantly higher than that of the siRNA control group(t=3.674, 6.375, P<0.05). After Cx43 overexpression, the proportion of early apoptosis and apoptosis combined with dead cells were significantly lower than that of vector control group(t=9.399, 11.190, P<0.05). The expression of cleaved caspase-3 in the Cx43 silencing group was higher than that in the siRNA control group, but this protein in the Cx43 overexpressed group was lower than that in the vector control group.ConclusionsCx43 may protect X-ray irradiated HUVEC cells from apoptosis by down-regulating the activation of caspase-3.Key words: X-rays; HUVEC cell; Cx43; Apoptosis; cleaved caspase-3" @default.
- W3029125761 created "2020-06-05" @default.
- W3029125761 creator A5010297642 @default.
- W3029125761 creator A5032447166 @default.
- W3029125761 creator A5053662941 @default.
- W3029125761 creator A5072743477 @default.
- W3029125761 creator A5079305110 @default.
- W3029125761 creator A5083507260 @default.
- W3029125761 date "2018-10-25" @default.
- W3029125761 modified "2023-09-28" @default.
- W3029125761 title "Role of connexin 43 in X-ray induced apoptosis in human umbilical vein endothelial cells and its mechanism" @default.
- W3029125761 doi "https://doi.org/10.3760/cma.j.issn.0254-5098.2018.10.002" @default.
- W3029125761 hasPublicationYear "2018" @default.
- W3029125761 type Work @default.
- W3029125761 sameAs 3029125761 @default.
- W3029125761 citedByCount "0" @default.
- W3029125761 crossrefType "journal-article" @default.
- W3029125761 hasAuthorship W3029125761A5010297642 @default.
- W3029125761 hasAuthorship W3029125761A5032447166 @default.
- W3029125761 hasAuthorship W3029125761A5053662941 @default.
- W3029125761 hasAuthorship W3029125761A5072743477 @default.
- W3029125761 hasAuthorship W3029125761A5079305110 @default.
- W3029125761 hasAuthorship W3029125761A5083507260 @default.
- W3029125761 hasConcept C104317684 @default.
- W3029125761 hasConcept C119056186 @default.
- W3029125761 hasConcept C153911025 @default.
- W3029125761 hasConcept C173396325 @default.
- W3029125761 hasConcept C185592680 @default.
- W3029125761 hasConcept C190283241 @default.
- W3029125761 hasConcept C202751555 @default.
- W3029125761 hasConcept C22615655 @default.
- W3029125761 hasConcept C2776415932 @default.
- W3029125761 hasConcept C2777411675 @default.
- W3029125761 hasConcept C54009773 @default.
- W3029125761 hasConcept C54355233 @default.
- W3029125761 hasConcept C553184892 @default.
- W3029125761 hasConcept C55493867 @default.
- W3029125761 hasConcept C81885089 @default.
- W3029125761 hasConcept C86803240 @default.
- W3029125761 hasConceptScore W3029125761C104317684 @default.
- W3029125761 hasConceptScore W3029125761C119056186 @default.
- W3029125761 hasConceptScore W3029125761C153911025 @default.
- W3029125761 hasConceptScore W3029125761C173396325 @default.
- W3029125761 hasConceptScore W3029125761C185592680 @default.
- W3029125761 hasConceptScore W3029125761C190283241 @default.
- W3029125761 hasConceptScore W3029125761C202751555 @default.
- W3029125761 hasConceptScore W3029125761C22615655 @default.
- W3029125761 hasConceptScore W3029125761C2776415932 @default.
- W3029125761 hasConceptScore W3029125761C2777411675 @default.
- W3029125761 hasConceptScore W3029125761C54009773 @default.
- W3029125761 hasConceptScore W3029125761C54355233 @default.
- W3029125761 hasConceptScore W3029125761C553184892 @default.
- W3029125761 hasConceptScore W3029125761C55493867 @default.
- W3029125761 hasConceptScore W3029125761C81885089 @default.
- W3029125761 hasConceptScore W3029125761C86803240 @default.
- W3029125761 hasIssue "10" @default.
- W3029125761 hasLocation W30291257611 @default.
- W3029125761 hasOpenAccess W3029125761 @default.
- W3029125761 hasPrimaryLocation W30291257611 @default.
- W3029125761 hasRelatedWork W2137420912 @default.
- W3029125761 hasRelatedWork W2160296373 @default.
- W3029125761 hasRelatedWork W2353315404 @default.
- W3029125761 hasRelatedWork W2355707334 @default.
- W3029125761 hasRelatedWork W2367982937 @default.
- W3029125761 hasRelatedWork W2372968129 @default.
- W3029125761 hasRelatedWork W2373814643 @default.
- W3029125761 hasRelatedWork W2376035407 @default.
- W3029125761 hasRelatedWork W2380401997 @default.
- W3029125761 hasRelatedWork W2381323543 @default.
- W3029125761 hasRelatedWork W2382325980 @default.
- W3029125761 hasRelatedWork W2382932134 @default.
- W3029125761 hasRelatedWork W2393539989 @default.
- W3029125761 hasRelatedWork W2394494455 @default.
- W3029125761 hasRelatedWork W2411193031 @default.
- W3029125761 hasRelatedWork W2413902205 @default.
- W3029125761 hasRelatedWork W2957414600 @default.
- W3029125761 hasRelatedWork W3031887293 @default.
- W3029125761 hasRelatedWork W3144197024 @default.
- W3029125761 hasRelatedWork W3149479528 @default.
- W3029125761 hasVolume "38" @default.
- W3029125761 isParatext "false" @default.
- W3029125761 isRetracted "false" @default.
- W3029125761 magId "3029125761" @default.
- W3029125761 workType "article" @default.