Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029144854> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W3029144854 endingPage "1074" @default.
- W3029144854 startingPage "1072" @default.
- W3029144854 abstract "ObjectiveTo investigate the impact of microRNA(miRNA)- 21 antisense oligonucleotide on cisplatin(DDP) resistance of EC9706/DDP cells.MethodsmiRNA- 21 antisense oligonucleotides were designed and transfected into EC9706/DDP- resistant cells.The miRNA- 21 expression levels were detected by real- time fluorescent quantitative polymerase chain reaction(FQ- PCR).Methyl thiazol tetrazolium(MTT) method was used to measure the sensitivity of cells to DDP drugs.Colony formation assay was used to test the cell proliferation. The change of apoptosis was examined by flow cytometry. Western blotting was performed to detect the expression of B cell lymphoma/leukemia- 2(bcl- 2), and bcl- 2 associated X protein(bax) proteins.ResultsAs compared with miRNA- NC negative control group and blank control group, the miRNA- 21 relative expression was decreased to(0.21±0.89),50% inhibitory dose(IC50)decreased to(16.22±1.85)mg/L, the number of clonal colonies was reduced, the apoptosis rate significantly increased to(30.5±2.9)%, bcl- 2 protein expression decreased,and bax protein expression increased in miRNA- 21 antisense oligonucleotide group(P< 0.05).ConclusionmiRNA - 21 decreased the resistance of EC9706/DDP cells to DDP possibly through reducing the bcl- 2 protein expression, and increasing the bax protein expression.Key words: MicroRNA-21; Esophageal cancer; Cisplatin" @default.
- W3029144854 created "2020-06-05" @default.
- W3029144854 creator A5069586733 @default.
- W3029144854 date "2015-05-08" @default.
- W3029144854 modified "2023-09-23" @default.
- W3029144854 title "Impact of microRNA- 21 antisense oligonucleotide on cisplatin resistance of EC9706/DDP cells" @default.
- W3029144854 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2015.05.043" @default.
- W3029144854 hasPublicationYear "2015" @default.
- W3029144854 type Work @default.
- W3029144854 sameAs 3029144854 @default.
- W3029144854 citedByCount "0" @default.
- W3029144854 crossrefType "journal-article" @default.
- W3029144854 hasAuthorship W3029144854A5069586733 @default.
- W3029144854 hasConcept C104317684 @default.
- W3029144854 hasConcept C129312508 @default.
- W3029144854 hasConcept C145059251 @default.
- W3029144854 hasConcept C153911025 @default.
- W3029144854 hasConcept C185592680 @default.
- W3029144854 hasConcept C190283241 @default.
- W3029144854 hasConcept C2776694085 @default.
- W3029144854 hasConcept C2778239845 @default.
- W3029144854 hasConcept C54009773 @default.
- W3029144854 hasConcept C54355233 @default.
- W3029144854 hasConcept C553184892 @default.
- W3029144854 hasConcept C55493867 @default.
- W3029144854 hasConcept C62112901 @default.
- W3029144854 hasConcept C81885089 @default.
- W3029144854 hasConcept C85265743 @default.
- W3029144854 hasConcept C86803240 @default.
- W3029144854 hasConceptScore W3029144854C104317684 @default.
- W3029144854 hasConceptScore W3029144854C129312508 @default.
- W3029144854 hasConceptScore W3029144854C145059251 @default.
- W3029144854 hasConceptScore W3029144854C153911025 @default.
- W3029144854 hasConceptScore W3029144854C185592680 @default.
- W3029144854 hasConceptScore W3029144854C190283241 @default.
- W3029144854 hasConceptScore W3029144854C2776694085 @default.
- W3029144854 hasConceptScore W3029144854C2778239845 @default.
- W3029144854 hasConceptScore W3029144854C54009773 @default.
- W3029144854 hasConceptScore W3029144854C54355233 @default.
- W3029144854 hasConceptScore W3029144854C553184892 @default.
- W3029144854 hasConceptScore W3029144854C55493867 @default.
- W3029144854 hasConceptScore W3029144854C62112901 @default.
- W3029144854 hasConceptScore W3029144854C81885089 @default.
- W3029144854 hasConceptScore W3029144854C85265743 @default.
- W3029144854 hasConceptScore W3029144854C86803240 @default.
- W3029144854 hasIssue "05" @default.
- W3029144854 hasLocation W30291448541 @default.
- W3029144854 hasOpenAccess W3029144854 @default.
- W3029144854 hasPrimaryLocation W30291448541 @default.
- W3029144854 hasRelatedWork W2000796581 @default.
- W3029144854 hasRelatedWork W2078016150 @default.
- W3029144854 hasRelatedWork W2090334688 @default.
- W3029144854 hasRelatedWork W2098602005 @default.
- W3029144854 hasRelatedWork W2125009611 @default.
- W3029144854 hasRelatedWork W2348262167 @default.
- W3029144854 hasRelatedWork W2351372612 @default.
- W3029144854 hasRelatedWork W2359515711 @default.
- W3029144854 hasRelatedWork W2361057567 @default.
- W3029144854 hasRelatedWork W2361121040 @default.
- W3029144854 hasRelatedWork W2367628546 @default.
- W3029144854 hasRelatedWork W2372192613 @default.
- W3029144854 hasRelatedWork W2379365134 @default.
- W3029144854 hasRelatedWork W2393746733 @default.
- W3029144854 hasRelatedWork W2414821628 @default.
- W3029144854 hasRelatedWork W2765786604 @default.
- W3029144854 hasRelatedWork W3029636759 @default.
- W3029144854 hasRelatedWork W3030154651 @default.
- W3029144854 hasRelatedWork W3031371406 @default.
- W3029144854 hasRelatedWork W64771371 @default.
- W3029144854 hasVolume "32" @default.
- W3029144854 isParatext "false" @default.
- W3029144854 isRetracted "false" @default.
- W3029144854 magId "3029144854" @default.
- W3029144854 workType "article" @default.