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• W3029168689 abstract "Objective The aim of this study was to explore the association between circ-CSPP1 and the proliferation, invasion, and migration of glioma cancer (GC). Patients and methods Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detected circ-CSPP1 expression in GC tissues and cells. Subsequently, siRNA was transfected to suppress circ-CSPP1 expression in vitro. Cell counting kit-8 (CCK-8) assay, colony formation assay, and 5-Ethynyl-2'-deoxyuridine (EdU) staining assay were performed to examine the proliferation of GC cells. Meanwhile, transwell assay was conducted to determine the invasion and migration of GC cells. Furthermore, Western blot assay was conducted to analyze the protein expressions of E-cadherin, N-cadherin, and Vimentin. Results Circ-CSPP1 expression was significantly up-regulated both in GC tissues and cells. GC cells with low expression of circ-CSPP1 showed significantly reduced proliferation, invasion, and migration abilities. In addition, up-regulated E-cadherin protein expression, along with down-regulated N-cadherin and Vimentin protein expressions were observed in GC cells with circ-CSPP1 siRNA treatment. Conclusions Circ-CSPP1 promoted the proliferation, invasion, and migration of GC cells." @default.
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• W3029168689 date "2020-05-01" @default.
• W3029168689 modified "2023-09-27" @default.
• W3029168689 title "Roles of circ-CSPP1 on the proliferation and metastasis of glioma cancer." @default.
• W3029168689 doi "https://doi.org/10.26355/eurrev_202005_21337" @default.
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