Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029175751> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W3029175751 endingPage "2240" @default.
- W3029175751 startingPage "2237" @default.
- W3029175751 abstract "ObjectiveTo study the effect of co-culture of tubal epithelial cells on the development of parthenogenetic embryos in vitro.MethodsRabbit oviduct epithelial cells were collected by trypsin and cultured at 37 ℃ and 5%CO2. Six to eight weeks age of kunming white female mice were controlled ovarian hyperstimulation. Mature oocytes were parthenogenetic activated by 7% ethanol and 5 mg/L cytochalasin B. Parthenogenetic activated eggs were cultured in the control group (simple culture), and the experimental group (rabbit oviduct epithelial cells P0 and P1, P2, P3, P4 co-culture system). After 1-5 d, the mice parthenogenetic embryos to 2-cell, 4-cell, 8-cell and the blastocyst were observed.ResultsCompared with the control group, the parthenogenetic activated eggs of mice were co-cultured with primary P0 and pass-through P1, P2 and P3 rabbit oviduct epithelial cells, and there was no significant difference in the formation rate of 2-cell and 4-cell (P>0.05). The 8-cell formation rate was significantly increased (57.5% vs. 80.2%, 85.0%, 81.8% vs. 76.8%, χ2=14.389, 23.803, 17.319, 10.078, P<0.01). Blastocyst formation rate increased significantly (34.5% vs. 57.9%, 62.9%, 58.3%, 50.4%, χ2=13.126, 20.094, 13.857, 6.116, P<0.05).ConclusionCo-culture of oviduct epithelial cells can obviously improve the development potential of parthenogenetic embryos in vitro.Key words: Parthenogenetic embryos; Co-culture; Blastocyst rate" @default.
- W3029175751 created "2020-06-05" @default.
- W3029175751 creator A5013135288 @default.
- W3029175751 creator A5022439109 @default.
- W3029175751 creator A5089725084 @default.
- W3029175751 date "2019-12-08" @default.
- W3029175751 modified "2023-09-26" @default.
- W3029175751 title "Effects of co-culture on in vitro development of parthenogenetic embryos in mice" @default.
- W3029175751 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2019.12.034" @default.
- W3029175751 hasPublicationYear "2019" @default.
- W3029175751 type Work @default.
- W3029175751 sameAs 3029175751 @default.
- W3029175751 citedByCount "0" @default.
- W3029175751 crossrefType "journal-article" @default.
- W3029175751 hasAuthorship W3029175751A5013135288 @default.
- W3029175751 hasAuthorship W3029175751A5022439109 @default.
- W3029175751 hasAuthorship W3029175751A5089725084 @default.
- W3029175751 hasConcept C103725303 @default.
- W3029175751 hasConcept C140737988 @default.
- W3029175751 hasConcept C154280991 @default.
- W3029175751 hasConcept C16685009 @default.
- W3029175751 hasConcept C185592680 @default.
- W3029175751 hasConcept C196843134 @default.
- W3029175751 hasConcept C202751555 @default.
- W3029175751 hasConcept C2777129507 @default.
- W3029175751 hasConcept C2778177303 @default.
- W3029175751 hasConcept C2779012151 @default.
- W3029175751 hasConcept C54355233 @default.
- W3029175751 hasConcept C71924100 @default.
- W3029175751 hasConcept C86803240 @default.
- W3029175751 hasConcept C87073359 @default.
- W3029175751 hasConcept C95444343 @default.
- W3029175751 hasConceptScore W3029175751C103725303 @default.
- W3029175751 hasConceptScore W3029175751C140737988 @default.
- W3029175751 hasConceptScore W3029175751C154280991 @default.
- W3029175751 hasConceptScore W3029175751C16685009 @default.
- W3029175751 hasConceptScore W3029175751C185592680 @default.
- W3029175751 hasConceptScore W3029175751C196843134 @default.
- W3029175751 hasConceptScore W3029175751C202751555 @default.
- W3029175751 hasConceptScore W3029175751C2777129507 @default.
- W3029175751 hasConceptScore W3029175751C2778177303 @default.
- W3029175751 hasConceptScore W3029175751C2779012151 @default.
- W3029175751 hasConceptScore W3029175751C54355233 @default.
- W3029175751 hasConceptScore W3029175751C71924100 @default.
- W3029175751 hasConceptScore W3029175751C86803240 @default.
- W3029175751 hasConceptScore W3029175751C87073359 @default.
- W3029175751 hasConceptScore W3029175751C95444343 @default.
- W3029175751 hasIssue "12" @default.
- W3029175751 hasLocation W30291757511 @default.
- W3029175751 hasOpenAccess W3029175751 @default.
- W3029175751 hasPrimaryLocation W30291757511 @default.
- W3029175751 hasRelatedWork W1991999667 @default.
- W3029175751 hasRelatedWork W1997868327 @default.
- W3029175751 hasRelatedWork W1999682658 @default.
- W3029175751 hasRelatedWork W2102541619 @default.
- W3029175751 hasRelatedWork W2153280033 @default.
- W3029175751 hasRelatedWork W2250163366 @default.
- W3029175751 hasRelatedWork W2312611421 @default.
- W3029175751 hasRelatedWork W233030934 @default.
- W3029175751 hasRelatedWork W2347243395 @default.
- W3029175751 hasRelatedWork W2351000880 @default.
- W3029175751 hasRelatedWork W2351966618 @default.
- W3029175751 hasRelatedWork W2359106214 @default.
- W3029175751 hasRelatedWork W2360090914 @default.
- W3029175751 hasRelatedWork W2369085533 @default.
- W3029175751 hasRelatedWork W2376501381 @default.
- W3029175751 hasRelatedWork W2390816472 @default.
- W3029175751 hasRelatedWork W2400664555 @default.
- W3029175751 hasRelatedWork W2599510831 @default.
- W3029175751 hasRelatedWork W2774837170 @default.
- W3029175751 hasRelatedWork W3119210994 @default.
- W3029175751 hasVolume "36" @default.
- W3029175751 isParatext "false" @default.
- W3029175751 isRetracted "false" @default.
- W3029175751 magId "3029175751" @default.
- W3029175751 workType "article" @default.