Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029202337> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W3029202337 endingPage "251" @default.
- W3029202337 startingPage "249" @default.
- W3029202337 abstract "ObjectiveTo observe the long non-coding RNA SPRY4-IT1 expression level in non-small cell lung cancer and its biological funciton in aspects of cell proliferation.MethodsReal-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to measure the expression of SPRY4-IT1 in 32 non-small cell lung cancer (NSCLC) patients. Loss of function approach was then applied to confirm the biological function, especially cell prolifieration in cultured human lung cancer cells A5549, by cell counting kit-8 (CCK-8) and clonogenic assay. We further used western blot to reveal the activation of zinc finger protein 703 (ZNF703) by SPRY4-IT1.ResultsThe SPRY4-IT1 relative expression level were 1.12±0.78 and 3.24±2.01 respectively for tumor tissue and norma tissue of NSCLC patients with statistical difference (t=5.56, P=0.001); SPRY4-IT1 expression level were significant decreased by transinfection of si-SPRY4-IT1 in A549 cells. A549 cell clone formation ability was significantly reduced by traninfection of si-SPRY4-IT1. The mRNA expression of ZNF703 were 0.61±0.07 and 0.96±0.08 for si-SPRY4-IT1 and si-nc A549 cells with statistical difference (P=0.001); The protein expression of ZNF703 were 0.08±0.02 and 0.12±0.03 for si-SPRY4-IT1 and si-nc A549 cells with statistical difference (P=0.001).ConclusionSPRY4-IT1 expression is up-regulated in tumor tissue of non-small cell lung cancer patients and the SPRY4-IT1/ZNF703 axis might contribute to increased proliferation ability of lung cancer cells.Key words: SPRY4-IT1; Non-small cell lung cancer; Long chain of non encoding RNA; Proliferation ability" @default.
- W3029202337 created "2020-06-05" @default.
- W3029202337 creator A5010520524 @default.
- W3029202337 creator A5026429908 @default.
- W3029202337 creator A5042651162 @default.
- W3029202337 creator A5051219791 @default.
- W3029202337 creator A5066028215 @default.
- W3029202337 creator A5079567542 @default.
- W3029202337 creator A5084190176 @default.
- W3029202337 date "2017-02-08" @default.
- W3029202337 modified "2023-09-23" @default.
- W3029202337 title "SPRY4-IT1 contributes to increased cell proliferation by activating zinc finger protein 703 expression in non-small cell lung cancer" @default.
- W3029202337 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2017.02.023" @default.
- W3029202337 hasPublicationYear "2017" @default.
- W3029202337 type Work @default.
- W3029202337 sameAs 3029202337 @default.
- W3029202337 citedByCount "0" @default.
- W3029202337 crossrefType "journal-article" @default.
- W3029202337 hasAuthorship W3029202337A5010520524 @default.
- W3029202337 hasAuthorship W3029202337A5026429908 @default.
- W3029202337 hasAuthorship W3029202337A5042651162 @default.
- W3029202337 hasAuthorship W3029202337A5051219791 @default.
- W3029202337 hasAuthorship W3029202337A5066028215 @default.
- W3029202337 hasAuthorship W3029202337A5079567542 @default.
- W3029202337 hasAuthorship W3029202337A5084190176 @default.
- W3029202337 hasConcept C104317684 @default.
- W3029202337 hasConcept C105580179 @default.
- W3029202337 hasConcept C117262875 @default.
- W3029202337 hasConcept C127561419 @default.
- W3029202337 hasConcept C143998085 @default.
- W3029202337 hasConcept C1491633281 @default.
- W3029202337 hasConcept C153911025 @default.
- W3029202337 hasConcept C24284526 @default.
- W3029202337 hasConcept C2776256026 @default.
- W3029202337 hasConcept C2776415932 @default.
- W3029202337 hasConcept C48023723 @default.
- W3029202337 hasConcept C502942594 @default.
- W3029202337 hasConcept C55493867 @default.
- W3029202337 hasConcept C62112901 @default.
- W3029202337 hasConcept C71924100 @default.
- W3029202337 hasConcept C81729549 @default.
- W3029202337 hasConcept C86339819 @default.
- W3029202337 hasConcept C86803240 @default.
- W3029202337 hasConceptScore W3029202337C104317684 @default.
- W3029202337 hasConceptScore W3029202337C105580179 @default.
- W3029202337 hasConceptScore W3029202337C117262875 @default.
- W3029202337 hasConceptScore W3029202337C127561419 @default.
- W3029202337 hasConceptScore W3029202337C143998085 @default.
- W3029202337 hasConceptScore W3029202337C1491633281 @default.
- W3029202337 hasConceptScore W3029202337C153911025 @default.
- W3029202337 hasConceptScore W3029202337C24284526 @default.
- W3029202337 hasConceptScore W3029202337C2776256026 @default.
- W3029202337 hasConceptScore W3029202337C2776415932 @default.
- W3029202337 hasConceptScore W3029202337C48023723 @default.
- W3029202337 hasConceptScore W3029202337C502942594 @default.
- W3029202337 hasConceptScore W3029202337C55493867 @default.
- W3029202337 hasConceptScore W3029202337C62112901 @default.
- W3029202337 hasConceptScore W3029202337C71924100 @default.
- W3029202337 hasConceptScore W3029202337C81729549 @default.
- W3029202337 hasConceptScore W3029202337C86339819 @default.
- W3029202337 hasConceptScore W3029202337C86803240 @default.
- W3029202337 hasIssue "2" @default.
- W3029202337 hasLocation W30292023371 @default.
- W3029202337 hasOpenAccess W3029202337 @default.
- W3029202337 hasPrimaryLocation W30292023371 @default.
- W3029202337 hasRelatedWork W2017128657 @default.
- W3029202337 hasRelatedWork W2365056729 @default.
- W3029202337 hasRelatedWork W2389089070 @default.
- W3029202337 hasRelatedWork W2419127979 @default.
- W3029202337 hasRelatedWork W2465098967 @default.
- W3029202337 hasRelatedWork W2742576530 @default.
- W3029202337 hasRelatedWork W2749889977 @default.
- W3029202337 hasRelatedWork W2795101768 @default.
- W3029202337 hasRelatedWork W2891835310 @default.
- W3029202337 hasRelatedWork W2903726061 @default.
- W3029202337 hasRelatedWork W2952657970 @default.
- W3029202337 hasRelatedWork W2954261580 @default.
- W3029202337 hasRelatedWork W2993472282 @default.
- W3029202337 hasRelatedWork W3014868668 @default.
- W3029202337 hasRelatedWork W3029607509 @default.
- W3029202337 hasRelatedWork W3030594824 @default.
- W3029202337 hasRelatedWork W3032564340 @default.
- W3029202337 hasRelatedWork W3032720311 @default.
- W3029202337 hasRelatedWork W3037919690 @default.
- W3029202337 hasRelatedWork W3088680939 @default.
- W3029202337 hasVolume "34" @default.
- W3029202337 isParatext "false" @default.
- W3029202337 isRetracted "false" @default.
- W3029202337 magId "3029202337" @default.
- W3029202337 workType "article" @default.