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- W3029203944 abstract "目的 通过不同剂量重组人骨形态发生蛋白-2(rhBMP-2)刺激原代培养的小鼠胚胎成骨细胞,探讨BMP-2对成骨细胞血管内皮生长因子(VEGF)表达的影响及规律.方法 取19 d胎龄的小鼠胚胎颅骨成骨细胞进行体外培养,采用RT-PCR法或Western blot法染色法检测不同浓度rhBMP-2、不同作用时间下对成骨细胞VEGF mRNA表达的影响及规律.结果 RT-PCR法检测到VEGF mRNA在原代成骨细胞内稳定表达;半定量分析表明,随着rhBMP-2浓度的升高,VEGF mRNA的表达量逐渐增加,当rhBMP-2浓度为300 ng/mL左右时,VEGF mRNA的表达量最高,超过此浓度,VEGF的表达则有下降趋势;应用终浓度rhBMP-2(300ng/mL)刺激成骨细胞时,不同作用时间下VEGF mRNA的表达量呈双时相,分别在3 h和24 h时出现高峰,48 h时VEGF mRNA的表达仍保持较高水平.除去各自对照组的影响,各时间点VEGF表达的相对值[(rhBMP-2组/对照组)比值]亦呈时间依赖性关系,6 h时明显升高为对照组的2.5倍(P＜0.05),36 h时约升高为对照组的2倍.结论 VEGF可在原代鼠胚成骨细胞内稳定表达;rhBMP-2在体外可刺激原代骨细胞VEGF的表达,并呈一定的浓度和时间依赖性关系。" @default.
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- W3029203944 date "2006-08-15" @default.
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- W3029203944 title "Effect of human bone morphogenetic protein-2 on vascular endothelial growth factor expression in fetal mouse osteoblasts" @default.
- W3029203944 doi "https://doi.org/10.3760/cma.j.issn.1671-7600.2006.08.015" @default.
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