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• W3029229212 abstract "RT-qPCR requires an adequate choice of stably expressed reference genes for accurate normalization of mRNA expression. However, testing a panel of reference genes is often time-consuming and expensive. In this work, we aimed to develop a set of multiplex real-time PCR assays for RT-qPCR analysis of commonly used housekeeping genes in laboratory rats. Using Hydrolysis probe (TaqMan®) technology, we have designed and optimized three triplex qPCR assays (Actb + Gapdh + B2m; Rpl13a + Sdha + Ppia; Hprt1+Pgk1+Ywhaz) demonstrating optimal PCR amplification efficiencies (from 94.7 to 100.5%) and repeatability. Novel assays allow expression analysis of 9 reference genes in 3 reactions making possible a more time-efficient choice of reference genes in RT-qPCR experiments in Wistar rats in comparison with widespread singleplex assays." @default.
• W3029229212 created "2020-06-05" @default.
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• W3029229212 date "2020-10-01" @default.
• W3029229212 modified "2023-10-02" @default.
• W3029229212 title "Multiplex qPCR assay for assessment of reference gene expression stability in rat tissues/samples" @default.
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• W3029229212 doi "https://doi.org/10.1016/j.mcp.2020.101611" @default.
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