Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029242210> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W3029242210 endingPage "208" @default.
- W3029242210 startingPage "202" @default.
- W3029242210 abstract "ObjectiveTo investigate the effect of brusatol on meiosis initiation and early oogenesis.MethodsMating was timed overnight and the appearance of the vaginal plug was considered as embryonic 0.5 d (E0.5) in the next morning. Female genital ridges (GRs) were collected from E11.5, E12.5, E13.5, E14.5 fetal mice. The protein and mRNA levels of nuclear factor-E2 related factor 2 (Nrf2) were then tested by qRT-PCR and Western blotting, respectively. The isolated female GRs (E12.5) were incubated with dimethyl sulfoxide (DMSO, control gorup), 10 nmol/L and 20 nmol/L of brusatol, 1 μmol/L of retinoic acid (RA), or 20 nmol/L of brusatol+1 μmol/L of RA for 48 h. Some of the treated GRs were used to analyzed the mRNA levels of the genes that related with Nrf2 pathway, meiosis initiation and RA pathway. The others were cultured for additional 10 d to test the primordial follicle formation by paraffin sectioning and HE staining.ResultsBrusatol treatment not only downregulated mRNA levels of meiosis genes such as Stra8, Sycp3 and Dazl(P<0.05), but also decreased the number of primordial follicles in the ovarian tissues (151.1±6.8 vs.109.5±12.2, P<0.01). The number of primordial forllicles was further decreased by an increased brusatol treatment (109.5±12.2 vs. 49.1±8.0, P<0.01). As the only inhibitor of Nrf2 pathway, brusatol was also confirmed to be effective in GR. Additional studies showed brusatol inhibited the expression of retinaldehyde dehydrogenases (P=0.001) and retinoic acid receptor α (P=0.049), and the inhibition of brusatol on Stra8, Sycp3 and Dazl expression was partially rescued by the addition of RA (P<0.01).ConclusionBrusatol regulated meiosis initiation and early oogenesis in female germ cell via inhibiting Nrf2 pathway.Key words: Nuclear factor-E2 related factor 2; Brusatol; Genital ridge; Retinoic acid; Mouse" @default.
- W3029242210 created "2020-06-05" @default.
- W3029242210 creator A5008022927 @default.
- W3029242210 creator A5008484460 @default.
- W3029242210 creator A5018388754 @default.
- W3029242210 creator A5086454498 @default.
- W3029242210 creator A5091525731 @default.
- W3029242210 date "2019-03-25" @default.
- W3029242210 modified "2023-09-23" @default.
- W3029242210 title "Brusatol regulates female mouse germ cell meiosis initiation and early oogenesis" @default.
- W3029242210 doi "https://doi.org/10.3760/cma.j.issn.2096-2916.2019.03.006" @default.
- W3029242210 hasPublicationYear "2019" @default.
- W3029242210 type Work @default.
- W3029242210 sameAs 3029242210 @default.
- W3029242210 citedByCount "0" @default.
- W3029242210 crossrefType "journal-article" @default.
- W3029242210 hasAuthorship W3029242210A5008022927 @default.
- W3029242210 hasAuthorship W3029242210A5008484460 @default.
- W3029242210 hasAuthorship W3029242210A5018388754 @default.
- W3029242210 hasAuthorship W3029242210A5086454498 @default.
- W3029242210 hasAuthorship W3029242210A5091525731 @default.
- W3029242210 hasConcept C104317684 @default.
- W3029242210 hasConcept C148196450 @default.
- W3029242210 hasConcept C153911025 @default.
- W3029242210 hasConcept C163949779 @default.
- W3029242210 hasConcept C16685009 @default.
- W3029242210 hasConcept C185592680 @default.
- W3029242210 hasConcept C196843134 @default.
- W3029242210 hasConcept C2776690073 @default.
- W3029242210 hasConcept C2781121885 @default.
- W3029242210 hasConcept C55493867 @default.
- W3029242210 hasConcept C71924100 @default.
- W3029242210 hasConcept C86803240 @default.
- W3029242210 hasConcept C95444343 @default.
- W3029242210 hasConceptScore W3029242210C104317684 @default.
- W3029242210 hasConceptScore W3029242210C148196450 @default.
- W3029242210 hasConceptScore W3029242210C153911025 @default.
- W3029242210 hasConceptScore W3029242210C163949779 @default.
- W3029242210 hasConceptScore W3029242210C16685009 @default.
- W3029242210 hasConceptScore W3029242210C185592680 @default.
- W3029242210 hasConceptScore W3029242210C196843134 @default.
- W3029242210 hasConceptScore W3029242210C2776690073 @default.
- W3029242210 hasConceptScore W3029242210C2781121885 @default.
- W3029242210 hasConceptScore W3029242210C55493867 @default.
- W3029242210 hasConceptScore W3029242210C71924100 @default.
- W3029242210 hasConceptScore W3029242210C86803240 @default.
- W3029242210 hasConceptScore W3029242210C95444343 @default.
- W3029242210 hasIssue "3" @default.
- W3029242210 hasLocation W30292422101 @default.
- W3029242210 hasOpenAccess W3029242210 @default.
- W3029242210 hasPrimaryLocation W30292422101 @default.
- W3029242210 hasRelatedWork W131676287 @default.
- W3029242210 hasRelatedWork W1504512890 @default.
- W3029242210 hasRelatedWork W1563513390 @default.
- W3029242210 hasRelatedWork W1586091975 @default.
- W3029242210 hasRelatedWork W1963673782 @default.
- W3029242210 hasRelatedWork W1971131878 @default.
- W3029242210 hasRelatedWork W1973190082 @default.
- W3029242210 hasRelatedWork W2001259416 @default.
- W3029242210 hasRelatedWork W2022913294 @default.
- W3029242210 hasRelatedWork W2044133276 @default.
- W3029242210 hasRelatedWork W2045822421 @default.
- W3029242210 hasRelatedWork W2049683651 @default.
- W3029242210 hasRelatedWork W2057343428 @default.
- W3029242210 hasRelatedWork W2083403128 @default.
- W3029242210 hasRelatedWork W2114992270 @default.
- W3029242210 hasRelatedWork W2144972094 @default.
- W3029242210 hasRelatedWork W2146295958 @default.
- W3029242210 hasRelatedWork W2219403205 @default.
- W3029242210 hasRelatedWork W2413023395 @default.
- W3029242210 hasRelatedWork W2418422929 @default.
- W3029242210 hasVolume "39" @default.
- W3029242210 isParatext "false" @default.
- W3029242210 isRetracted "false" @default.
- W3029242210 magId "3029242210" @default.
- W3029242210 workType "article" @default.