Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029261567> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3029261567 endingPage "316" @default.
- W3029261567 startingPage "312" @default.
- W3029261567 abstract "ObjectiveTo develop a quick-convenient method for the titer determination of enterovirus 71 (EV71) .MethodsThe traditional method TCID50 was used to determine the sample titer of EV71 as a reference. The reference and other samples were re-tested by quantitative RT-PCR. The EV71 titer was calculated by Ct values of the reference and EV71 samples. The specificity and repeatability of this method was verified.ResultsThe detecting approach could be used for quick-convenient titer detection of EV71. RT-PCR showed a good linearity with the testing virus titer lgTCID50 range of 2.61-8.61 and correlation coefficient (R2) of 0.9881. The method had good specificity and repeatability, and was well consistent with TCID50 method with the coefficient of variation (CV) <5%.ConclusionsThe developed quantitative RT-PCR method shows a wide linear range, high specificity and superior repeatability. It can be a quick-convenient method for titer determination of EV71.Key words: Reverse transcriptase polymerase chain reaction; Enterovirus 71; Virus titer" @default.
- W3029261567 created "2020-06-05" @default.
- W3029261567 creator A5007802505 @default.
- W3029261567 creator A5018322243 @default.
- W3029261567 creator A5024534066 @default.
- W3029261567 creator A5027810506 @default.
- W3029261567 creator A5051994269 @default.
- W3029261567 creator A5082511360 @default.
- W3029261567 creator A5084714753 @default.
- W3029261567 date "2017-10-25" @default.
- W3029261567 modified "2023-09-26" @default.
- W3029261567 title "Quantitative reverse transcriptase PCR for quick titer determination of enterovirus 71" @default.
- W3029261567 doi "https://doi.org/10.3760/cma.j.issn.1673-4149.2017.05.006" @default.
- W3029261567 hasPublicationYear "2017" @default.
- W3029261567 type Work @default.
- W3029261567 sameAs 3029261567 @default.
- W3029261567 citedByCount "0" @default.
- W3029261567 crossrefType "journal-article" @default.
- W3029261567 hasAuthorship W3029261567A5007802505 @default.
- W3029261567 hasAuthorship W3029261567A5018322243 @default.
- W3029261567 hasAuthorship W3029261567A5024534066 @default.
- W3029261567 hasAuthorship W3029261567A5027810506 @default.
- W3029261567 hasAuthorship W3029261567A5051994269 @default.
- W3029261567 hasAuthorship W3029261567A5082511360 @default.
- W3029261567 hasAuthorship W3029261567A5084714753 @default.
- W3029261567 hasConcept C104317684 @default.
- W3029261567 hasConcept C154020017 @default.
- W3029261567 hasConcept C156719811 @default.
- W3029261567 hasConcept C159047783 @default.
- W3029261567 hasConcept C185592680 @default.
- W3029261567 hasConcept C2522874641 @default.
- W3029261567 hasConcept C2777888124 @default.
- W3029261567 hasConcept C2778889330 @default.
- W3029261567 hasConcept C32611913 @default.
- W3029261567 hasConcept C43617362 @default.
- W3029261567 hasConcept C48023723 @default.
- W3029261567 hasConcept C49105822 @default.
- W3029261567 hasConcept C55493867 @default.
- W3029261567 hasConcept C86803240 @default.
- W3029261567 hasConcept C89838059 @default.
- W3029261567 hasConceptScore W3029261567C104317684 @default.
- W3029261567 hasConceptScore W3029261567C154020017 @default.
- W3029261567 hasConceptScore W3029261567C156719811 @default.
- W3029261567 hasConceptScore W3029261567C159047783 @default.
- W3029261567 hasConceptScore W3029261567C185592680 @default.
- W3029261567 hasConceptScore W3029261567C2522874641 @default.
- W3029261567 hasConceptScore W3029261567C2777888124 @default.
- W3029261567 hasConceptScore W3029261567C2778889330 @default.
- W3029261567 hasConceptScore W3029261567C32611913 @default.
- W3029261567 hasConceptScore W3029261567C43617362 @default.
- W3029261567 hasConceptScore W3029261567C48023723 @default.
- W3029261567 hasConceptScore W3029261567C49105822 @default.
- W3029261567 hasConceptScore W3029261567C55493867 @default.
- W3029261567 hasConceptScore W3029261567C86803240 @default.
- W3029261567 hasConceptScore W3029261567C89838059 @default.
- W3029261567 hasIssue "5" @default.
- W3029261567 hasLocation W30292615671 @default.
- W3029261567 hasOpenAccess W3029261567 @default.
- W3029261567 hasPrimaryLocation W30292615671 @default.
- W3029261567 hasRelatedWork W2009338900 @default.
- W3029261567 hasRelatedWork W2019432208 @default.
- W3029261567 hasRelatedWork W2069219584 @default.
- W3029261567 hasRelatedWork W2162170062 @default.
- W3029261567 hasRelatedWork W2247782372 @default.
- W3029261567 hasRelatedWork W2348568545 @default.
- W3029261567 hasRelatedWork W2350153554 @default.
- W3029261567 hasRelatedWork W2354521436 @default.
- W3029261567 hasRelatedWork W2372110214 @default.
- W3029261567 hasRelatedWork W2374200857 @default.
- W3029261567 hasRelatedWork W2385300319 @default.
- W3029261567 hasRelatedWork W2387450218 @default.
- W3029261567 hasRelatedWork W2393349495 @default.
- W3029261567 hasRelatedWork W2414789208 @default.
- W3029261567 hasRelatedWork W2480913168 @default.
- W3029261567 hasRelatedWork W2743863637 @default.
- W3029261567 hasRelatedWork W3042837278 @default.
- W3029261567 hasRelatedWork W3186753913 @default.
- W3029261567 hasRelatedWork W3203204494 @default.
- W3029261567 hasRelatedWork W3207839323 @default.
- W3029261567 hasVolume "44" @default.
- W3029261567 isParatext "false" @default.
- W3029261567 isRetracted "false" @default.
- W3029261567 magId "3029261567" @default.
- W3029261567 workType "article" @default.