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• W3029265374 abstract "ObjectiveTo study the effect of inhibiting glycogen synthase kinase3β (GSK3β) on cell proliferation and epithelial mesenchymal transition (EMT) in pancreatic cancer PANC1 cells.MethodsThe siRNA trageting GSK3β(siRNA-GSK3β) and negative control siRNA (siRNA-NC) were synthesized to transfect pancreatic cancer PANC1 cells. MTT assay was used to detect cell proliferation. mRNA expression of Wnt, β-catenin, C-myc, CyclinD1 in Wnt/β-catenin signaling, Shh and Smo in Hedgehog pathway, PI3K, Akt, mTOR, 4E-BP1, p70S6K in PI3K/Akt/mTOR signal pathway and Slug, Snail, Twist, E-cadherin, N-cadherin, Vimentin involved in EMT were detected by RT-PCR.ResultsGSK3β mRNA expression was 1 in cells transfected with siRNA-NC, which was 0.25±0.08, 0.62±0.09, 0.70±0.11 in cells with 3 different siRNA-GSK3βs, respectively. GSK3β mRNA was significantly decreased by transfection of siRNA-GSK3β(P<0.0001). The cell proliferation of siRNA-GSK3β group was significantly lower than that of siRNA-NC group(P<0.05). Wnt, β-catenin, C-myc, CyclinD1, PI3K, Akt, mTOR, 4E-BP1, p70S6K, Slug, Snail, N-cadherin, Vimentin mRNA in cells transfected with siRNA-GSK3β was 0.28±0.04, 0.47±0.05, 0.37±0.05, 0.62±0.08, 0.22±0.03, 0.47±0.06, 0.65±0.08, 0.39±0.04, 0.56±0.07, 0.33±0.05, 0.46±0.07, 0.55±0.06, 0.38±0.04, which were greatly lower than those in cells transfected with siRNA-NC, and the difference was statistically significant (all P<0.05). Shh and Smo mRNA was 1.10±0.13 and 1.05±0.11, which was comparable with those in cells with siRNA-NC. Twist mRNA was 0.62±0.08, which was lower than that in cells with siRNA-NC, while E-cadherin mRNA was 2.12±0.25, which was higher than that in cells with siRNA-NC, but the differences were not statistically significant.ConclusionsGSK3β silencing in PANC1 cells can suppress cell proliferation and EMT of pancreatic cancer cells by regulating Wnt/β-catenin and PI3K/Akt pathway.Key words: Pancreatic neoplasms; Glycogen synthase kinase 3β; Cell proliferation; Epithelial mesenchymal transition" @default.
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• W3029265374 date "2016-04-20" @default.
• W3029265374 modified "2023-09-26" @default.
• W3029265374 title "Effects of glycogen synthase kinase3β silencing on cell growth and epithelial-mesenchymal transition in pancreatic cancer PANC1 cells" @default.
• W3029265374 doi "https://doi.org/10.3760/cma.j.issn.1674-1935.2016.02.002" @default.
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