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- W3029349054 abstract "Objective As generally known that non-cytotoxic pro-drug can be transformed into cytotoxic drug by suicide gene,this work is to investigate the effect of Coli cytosine deaminase/5-fluorocytosine suicide gene(CD/5-FC) used alone or combined with irradiation in esophageal carcinoma cell line(EC).Methods CD gene was amplified from Coli DNA genome library with PCR technique,with the eukaryotic vector pcDNA3.1-CD then constructed.EC109 cells were transfected with pcDNA3.1-CD by liposome method.The cytotoxic effect,by-stander effect and radiosensitization effect of CD/5-FC in EC109 was analyzed.Results The transfection of CD gene into EC109 and its transcription was confirmed by RT-PCR method.In vitro,5-FC showed significantly cytotoxic effect on the EPC cell transfected with CD gene.After adding 5-FC,the survival rate of cultured cell containing 5% transfect CD gene cell was 41.8%±14.2% while that in the control group was(94.6)±4.3%,(t=3.14,P0.05).The survival rate of cultured cell containing 10% transfected CD gene cell was 37.8±(4.4%) compared to 95.6%±5.4% in the control group,(t=9.75,P0.01).CD/5-FC showed significant radiosensitization effect,the survival fraction of CD transfected cell was much lower in 5-FC combined with irradiation,when compared with 5-FC alone and radiotherapy alone group together,(F=11.50,P0.01).When it was compared with 5-FC alone group and irradiation alone group separately,the difference was also significant(F=(4.11),P0.05 and F10.53,P0.01,respectively).Conclusions Suicide gene CD/5-FC shows conspicuous by-stander effect and radiosensitization effect." @default.
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- W3029349054 date "2005-09-15" @default.
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- W3029349054 title "Effect of suicidal gene combined with irradiation on esophageal carcinoma cell line" @default.
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