Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029381021> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W3029381021 endingPage "219" @default.
- W3029381021 startingPage "216" @default.
- W3029381021 abstract "ObjectiveTo establish and evaluate a Metapneumovirus real-time quantitative reverse transcription polymerase chain reaction (RT-PCR), detect clinical specimens and explore the clinical prevalence characteristics of Metapneumovirus-infected children.MethodsThe primers and probes which targeted the conserved genes F were designed, RNA standards were prepared to establish a standard curve, the sensitivity, specificity and reproducibility had been tested. 222 lower respiratory clinical specimens were collected from children in Lanzhou area with ARI. Metapneumovirus and co-infection viruses were detected simultaneously; further Metapneumovirus related epidemiology was studied.ResultsMetapneumovirus linear detection range was 10-108 copies/μl, the lowest detection limit was 10 copies/μl, the correlation coefficient was 1, the amplification efficiency was 91.62%, the CV of Ct value was less than 2%. Take conventional PCR product sequence results as reference, real-time quantitative RT-PCR sensitivity and specificity were 100% and 96.17%. Metapneumovirus detection rate were 9.46%, 13 cases for boys (5.86%), 8 case for girls (3.60%). The detection rate of spring, summer, autumn and winter were 15.71%, 0%, 5.08%, 11.11%. There were no significant differences between the Metapneumovirus viral load and mixed infection or the types of disease, clinical symptoms.ConclusionsMetapneumovirus real-time quantitative RT-PCR has been confirmed as a sensitive and specific method. Metapneumovirus was an important agent of children respiratory tract infection in Lanzhou region.We should take the long-term systematic surveillance seriously.Key words: Real-time quantitative reverse transcription PCR; Metapneumovirus" @default.
- W3029381021 created "2020-06-05" @default.
- W3029381021 creator A5017255280 @default.
- W3029381021 creator A5031371726 @default.
- W3029381021 creator A5046212411 @default.
- W3029381021 creator A5077256359 @default.
- W3029381021 date "2016-04-30" @default.
- W3029381021 modified "2023-10-09" @default.
- W3029381021 title "Establishment and application of Real-time quantitative PCR for human Metapneumovirus detection" @default.
- W3029381021 doi "https://doi.org/10.3760/cma.j.issn.1003-9279.2016.02.026" @default.
- W3029381021 hasPublicationYear "2016" @default.
- W3029381021 type Work @default.
- W3029381021 sameAs 3029381021 @default.
- W3029381021 citedByCount "0" @default.
- W3029381021 crossrefType "journal-article" @default.
- W3029381021 hasAuthorship W3029381021A5017255280 @default.
- W3029381021 hasAuthorship W3029381021A5031371726 @default.
- W3029381021 hasAuthorship W3029381021A5046212411 @default.
- W3029381021 hasAuthorship W3029381021A5077256359 @default.
- W3029381021 hasConcept C104317684 @default.
- W3029381021 hasConcept C105580179 @default.
- W3029381021 hasConcept C126322002 @default.
- W3029381021 hasConcept C159047783 @default.
- W3029381021 hasConcept C2776012195 @default.
- W3029381021 hasConcept C2780475896 @default.
- W3029381021 hasConcept C2911218186 @default.
- W3029381021 hasConcept C4692481 @default.
- W3029381021 hasConcept C48023723 @default.
- W3029381021 hasConcept C534529494 @default.
- W3029381021 hasConcept C55493867 @default.
- W3029381021 hasConcept C71924100 @default.
- W3029381021 hasConcept C86803240 @default.
- W3029381021 hasConceptScore W3029381021C104317684 @default.
- W3029381021 hasConceptScore W3029381021C105580179 @default.
- W3029381021 hasConceptScore W3029381021C126322002 @default.
- W3029381021 hasConceptScore W3029381021C159047783 @default.
- W3029381021 hasConceptScore W3029381021C2776012195 @default.
- W3029381021 hasConceptScore W3029381021C2780475896 @default.
- W3029381021 hasConceptScore W3029381021C2911218186 @default.
- W3029381021 hasConceptScore W3029381021C4692481 @default.
- W3029381021 hasConceptScore W3029381021C48023723 @default.
- W3029381021 hasConceptScore W3029381021C534529494 @default.
- W3029381021 hasConceptScore W3029381021C55493867 @default.
- W3029381021 hasConceptScore W3029381021C71924100 @default.
- W3029381021 hasConceptScore W3029381021C86803240 @default.
- W3029381021 hasIssue "2" @default.
- W3029381021 hasLocation W30293810211 @default.
- W3029381021 hasOpenAccess W3029381021 @default.
- W3029381021 hasPrimaryLocation W30293810211 @default.
- W3029381021 hasRelatedWork W2032933202 @default.
- W3029381021 hasRelatedWork W2040630586 @default.
- W3029381021 hasRelatedWork W2046973103 @default.
- W3029381021 hasRelatedWork W2120477426 @default.
- W3029381021 hasRelatedWork W2133406965 @default.
- W3029381021 hasRelatedWork W2137377471 @default.
- W3029381021 hasRelatedWork W2157699584 @default.
- W3029381021 hasRelatedWork W2351909659 @default.
- W3029381021 hasRelatedWork W2368150673 @default.
- W3029381021 hasRelatedWork W2369752506 @default.
- W3029381021 hasRelatedWork W2384180743 @default.
- W3029381021 hasRelatedWork W2392529619 @default.
- W3029381021 hasRelatedWork W2435846065 @default.
- W3029381021 hasRelatedWork W2601093417 @default.
- W3029381021 hasRelatedWork W2899088122 @default.
- W3029381021 hasRelatedWork W3030630290 @default.
- W3029381021 hasRelatedWork W3031129674 @default.
- W3029381021 hasRelatedWork W3031523426 @default.
- W3029381021 hasRelatedWork W3032776600 @default.
- W3029381021 hasRelatedWork W3210075144 @default.
- W3029381021 hasVolume "30" @default.
- W3029381021 isParatext "false" @default.
- W3029381021 isRetracted "false" @default.
- W3029381021 magId "3029381021" @default.
- W3029381021 workType "article" @default.