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- W3029387225 abstract "Objective To establish a highly specific and sensitive sandwich ELISA kit for detection of E. coli cell proteins. Methods E. coli cell proteins were used for immunization of rabbits and high titer antisera were obtained. The polyclonal antibodies(PAbs)against E. coli cell proteins were purified by saturated ammonium sulfate salting-out, anion exchange chromatography and affinity chromatography. The purified PAbs were used as coating antibodies, and biotin-labeled PAbs plus horseradish peroxidase-conjugated avidin were used as detecting antibodies. Results The sensitivity and detection range of the sandwich ELISA system for detection of E. coli cell proteins were 0.32 μg/L and 1-100 μg/L, respectively, and equal to the similar commercial kits in the world. This ELISA kit had good stability, and its intra- and inter-assay variation coefficients were less than 7.7% and less than 6. 2%, respectively. Conclusions The sandwich ELISA kit with high specificity, sensitivity and stability is established successfully and provides an important method for quality control of residual E. coli cell proteins in biological products.Key words: Escherichia coli; Enzyme-linked immunosorbent assay; Proteins; Polyclonal antibodies" @default.
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- W3029387225 date "2010-12-10" @default.
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- W3029387225 title "Establishment and application of sandwich ELISA kit for detection of E. coli cell proteins" @default.
- W3029387225 doi "https://doi.org/10.3760/cma.j.issn.1673-4211.2010.06.001" @default.
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