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• W3029388567 abstract "ObjectiveTo investigate the inhibitory role of curcumin in palmitic acid-induced inflammatory response of macrophages.MethodsMurine macrophage cell line RAW264.7 were treated with 0, 250, 500 μmol/L palmitic acid for 24 h, then the morphology of cells were observed, receptor interacting protein140( RIP140), tumor necrosis factor α(TNF-α) and interleukin 6(IL-6) mRNA levels were determined by reverse transcription-polymerase chain reaction (RT-PCR) and levels of TNF-α and IL-6 in supernatant were tested by enzyme- linked immunosorbent assay (ELISA). The optimum time and concentration of curcumin on RAW264.7 cells were determined by MTT assay. Cells were separated into two groups and treated with 20 μmol/L curcumin and the same volume of dimethyl sulfoxide (DMSO) for 1 h, respectively; then both groups were treated with 500 μmol/L palmitic acid. After 24 h, the morphology of cells were observed, and the mRNA abundance of RIP140, TNF-α and IL-6 and protein levels of TNF-α, IL-6 in supernatant were detected. The research data was assessed by one-way ANOWA and t test.ResultsAfter intervening with 500 μmol/L palmitic acid for 24 h, RIP140 mRNA level was higher than that in 0 μmol/L palmitic acid group (3.40±0.51 vs 1.01±0.21, t=7.436, P<0.01). After intervening with 0, 250, 500 μmol/L palmitic acid for 24 h, TNF-α mRNA levels (1.00±0.03, 1.79±0.12, 2.16±0.13) and protein levels ((197±25), (371±10), (485±17) ng/L), IL-6 mRNA levels (1.00±0.51, 2.55±0.15, 2.59±0.17) and protein levels ((953±66), (1081±36), (1182±18)ng/L) were increased significantly in a dose-dependent manner(F=99.308, 187.049, 152.958, 26.594, respectively, all P<0.01); After the treatment of palmitic acid, cells shrank, rounded and even disintegrated; The optimum time of curcumin treating on RAW264.7 cells was 1 h, and the optimum concentration was 20 μmol/L. After treated with 20 μmol/L curumin, compared to control group, RIP140 mRNA abundance (0.63±0.01 vs 1.00±0.05, t=-13.79, P<0.01), TNF-α mRNA abundance (0.64±0.11 vs 1.00±0.07, t=-4.532, P<0.05) and protein concentration ((322±12) vs (485±17) ng/L, t=-13.577, P<0.01), IL-6 mRNA abundance (0.57±0.05 vs 1.00±0.02, t=-14.167, P<0.01) and protein concentration ((241±47) vs (1182±18) ng/L, t=-44.810, P<0.01) were significantly lower, the morphology of cells was not impaired and cell junction still existed.ConclusionsCurcumin may repress palmitic acid-induced inflammation by decreasing RIP140 expression.Key words: Macrophages; Curcumin; Palmitic acid; Inflammatory response" @default.
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• W3029388567 date "2013-01-27" @default.
• W3029388567 modified "2023-09-28" @default.
• W3029388567 title "Inhibitory role of curcumin in palmitic acid-induced inflammatory response of macrophages" @default.
• W3029388567 doi "https://doi.org/10.3760/cma.j.issn.1674-5809.2013.01.011" @default.
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