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- W3029470003 abstract "ObjectiveTo explore whether receptor interacting protein kinase 1 (RIP1)/RIP3 pathways participate in hemin induced cell death in HT-22 hippocampal neuronal cells and investigate the potential neuroprotection of necrostatin-1 in hemin induced cell death in HT-22.MethodsFirst, different concentrations of hemin (0, 25, 50, 100 μmol/L) were added to HT-22 cells. Propidium iodide (PI) positive cells and cell viability were measured at 24 h after hemin treatment. Then, necrostatin-1, zVAD and reactive oxygen species (ROS) scavenger butylated hydroxyanisole (BHA) were applied to hemin-treated HT-22 cells. PI+ cells and cell viability were measured at 24 h after hemin treatment. MitoSox Red was used to indicate ROS level. Last, the effect of RIP3 in hemin induced HT-22 cell death was explored through RIP3 knockdown using siRNA. PI+ cells, cell viability and ROS lever were measured at 24 h after hemin treatment.ResultsHemin could induce a dose dependent cell death in HT22 neural cells. RIP1 specific inhibitor necrostatin-1 significantly inhibited cell death induced by hemin in HT-22 cells, greatly reducing PI+ cells, dramatically improving cell viability and decreasing ROS accumulation. BHA could significantly inhibit PI+ cells induced by hemin in HT-22 cells. Furthermore, silencing of RIP3 using siRNA attenuated hemin induced cell death in HT-22 cells, greatly reducing PI+ cells, dramatically improving cell viability and decreasing ROS accumulation.ConclusionThese data revealed that RIP1/ RIP3 pathway and ROS might mediate hemin induced cell death in HT-22 hippocampus neural cells, and necrostatin-1 played a neuroprotection role in hemin induced cell death in HT-22.Key words: Hemin; Necroptosis; Receptor interacting protein kinase 1; Receptor interacting protein kinase 3; Necrostatin-1" @default.
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- W3029470003 date "2018-10-15" @default.
- W3029470003 modified "2023-09-27" @default.
- W3029470003 title "Receptor interacting protein kinases 1 and 3 mediate hemin induced cell death in HT-22 hippocampal neuronal cells" @default.
- W3029470003 doi "https://doi.org/10.3877/cma.j.issn.2095-9141.2018.05.008" @default.
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