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- W3029563405 abstract "Japanese encephalitis virus (JEV) is one of the major causes of viral encephalitis all around the globe. Approximately 3 billion people in endemic areas are at risk of Japanese encephalitis. To develop a wholistic understanding of the viral proteome, it is important to investigate both its ordered and disordered proteins. However, the functional and structural significance of disordered regions in the JEV proteome has not been systematically investigated as of yet. To fill this gap, we used here a set of bioinformatics tools to analyze the JEV proteome for the predisposition of its proteins for intrinsic disorder and for the presence of the disorder‐based binding regions (also known as molecular recognition features, MoRFs). We also analyzed all JEV proteins for the presence of the probable nucleic acid‐binding (DNA and RNA) sites. The results of these computational studies are experimentally validated using JEV capsid protein as an illustrative example. In agreement with bioinformatic analysis, we found that the N‐terminal region of the JEV capsid (residues 1–30) is intrinsically disordered. We showed that this region is characterized by the temperature response typical for highly disordered proteins. Furthermore, we have experimentally shown that this disordered N‐terminal domain of a capsid protein has a noticeable ‘gain‐of‐structure’ potential. In addition, using DOPS liposomes, we demonstrated the presence of pronounced membrane‐mediated conformational changes in the N‐terminal region of JEV capsid. In our view, this disorder‐centric analysis would be helpful for a better understanding of the JEV pathogenesis." @default.
- W3029563405 created "2020-06-05" @default.
- W3029563405 creator A5011017617 @default.
- W3029563405 creator A5052775267 @default.
- W3029563405 creator A5062344898 @default.
- W3029563405 creator A5068982877 @default.
- W3029563405 creator A5075387650 @default.
- W3029563405 creator A5083199032 @default.
- W3029563405 creator A5090133974 @default.
- W3029563405 date "2020-06-18" @default.
- W3029563405 modified "2023-09-29" @default.
- W3029563405 title "Japanese encephalitis virus – exploring the dark proteome and disorder–function paradigm" @default.
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