FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3029568956> ?p ?o ?g. }

• W3029568956 endingPage "630" @default.
• W3029568956 startingPage "626" @default.
• W3029568956 abstract "ObjectiveTo investigate the role of Staphylococcus aureus enterotoxin B (SEB) in non-IgE mediated activation of mast cells (MCs) by in vitro co-culture of laboratory of allergic disease 2 (LAD2) cells and SEB.MethodsThe LAD2 cells were incubated with SEB at different concentrations of 0.01, 0.1, 1, 10 and 100 μg/ml, A23187 positive control and negative control separately for 30 minutes. Then, effects of SEB on the morphology of MCs were observed by using a light microscope, and culture supernatants of the above incubation systems were collected. The concentration of tryptase released from MCs was analyzed by enzymatic activity assay, and the level of histamine was detected by enzyme-linked immunosorbent assay (ELISA) .ResultsAfter 30-minute co-culture of LAD2 cells and SEB, MCs showed larger size, obscure boundaries, increased number of protuberances on the cell surface and decreased refractivity, with a radial burr fin-like appearance. After 30-minute co-culture of LAD2 cells and SEB at different concentrations of 0.01, 0.1, 1, 10 and 100 μg/ml, the concentrations of tryptase in the culture supernatants were 4.116 ± 0.651, 5.344 ± 0.874, 3.806 ± 0.459, 1.309 ± 0.247, 0.310 ± 0.199 ng/ml respectively. Additionally, the tryptase levels were significantly higher in the 0.01-, 0.1-, 1-μg/ml SEB groups than in the negative control group (1.538 ± 0.490, all P < 0.05) , and gradually decreased along with the increase of SEB concentrations. The histamine levels in the 0.01-, 0.1-, 1-, 10-and 100-μg/ml SEB groups were 242.409 ± 63.915, 522.491 ± 73.466, 550.926 ± 84.466, 334.397 ± 33.640, 226.527 ± 5.678 ng/ml respectively. In the 0.01-, 0.1-, 1-μg/ml SEB groups, the levels of histamine released from MCs were gradually increased along with the increase of SEB concentrations, and were significantly higher than those in the negative control group (146.436 ± 3.100, all P < 0.05) . However, with the continued increase of SEB concentrations, the histamine levels gradually decreased.ConclusionSEB can directly activate MCs by a non-IgE mediated mechanism, followed by morphologic changes of MCs and release of tryptase and histamine.Key words: Mast cells; Staphylococcus aureus; Enterotoxins; Tryptases; Histamine; Dermatitis, atopic" @default.
• W3029568956 created "2020-06-05" @default.
• W3029568956 creator A5027182487 @default.
• W3029568956 creator A5044622075 @default.
• W3029568956 creator A5053287115 @default.
• W3029568956 creator A5063391448 @default.
• W3029568956 date "2017-09-15" @default.
• W3029568956 modified "2023-09-24" @default.
• W3029568956 title "Role of Staphylococcus aureus enterotoxin B in activation of LAD2 mast cells" @default.
• W3029568956 doi "https://doi.org/10.3760/cma.j.issn.0412-4030.2017.09.002" @default.
• W3029568956 hasPublicationYear "2017" @default.
• W3029568956 type Work @default.
• W3029568956 sameAs 3029568956 @default.
• W3029568956 citedByCount "0" @default.
• W3029568956 crossrefType "journal-article" @default.
• W3029568956 hasAuthorship W3029568956A5027182487 @default.
• W3029568956 hasAuthorship W3029568956A5044622075 @default.
• W3029568956 hasAuthorship W3029568956A5053287115 @default.
• W3029568956 hasAuthorship W3029568956A5063391448 @default.
• W3029568956 hasConcept C104317684 @default.
• W3029568956 hasConcept C1122143 @default.
• W3029568956 hasConcept C141105273 @default.
• W3029568956 hasConcept C153911025 @default.
• W3029568956 hasConcept C159654299 @default.
• W3029568956 hasConcept C185592680 @default.
• W3029568956 hasConcept C202751555 @default.
• W3029568956 hasConcept C203014093 @default.
• W3029568956 hasConcept C25642318 @default.
• W3029568956 hasConcept C2779489039 @default.
• W3029568956 hasConcept C2779726688 @default.
• W3029568956 hasConcept C2781406822 @default.
• W3029568956 hasConcept C36333154 @default.
• W3029568956 hasConcept C523546767 @default.
• W3029568956 hasConcept C54355233 @default.
• W3029568956 hasConcept C547475151 @default.
• W3029568956 hasConcept C55493867 @default.
• W3029568956 hasConcept C66999527 @default.
• W3029568956 hasConcept C86803240 @default.
• W3029568956 hasConcept C89423630 @default.
• W3029568956 hasConcept C98274493 @default.
• W3029568956 hasConceptScore W3029568956C104317684 @default.
• W3029568956 hasConceptScore W3029568956C1122143 @default.
• W3029568956 hasConceptScore W3029568956C141105273 @default.
• W3029568956 hasConceptScore W3029568956C153911025 @default.
• W3029568956 hasConceptScore W3029568956C159654299 @default.
• W3029568956 hasConceptScore W3029568956C185592680 @default.
• W3029568956 hasConceptScore W3029568956C202751555 @default.
• W3029568956 hasConceptScore W3029568956C203014093 @default.
• W3029568956 hasConceptScore W3029568956C25642318 @default.
• W3029568956 hasConceptScore W3029568956C2779489039 @default.
• W3029568956 hasConceptScore W3029568956C2779726688 @default.
• W3029568956 hasConceptScore W3029568956C2781406822 @default.
• W3029568956 hasConceptScore W3029568956C36333154 @default.
• W3029568956 hasConceptScore W3029568956C523546767 @default.
• W3029568956 hasConceptScore W3029568956C54355233 @default.
• W3029568956 hasConceptScore W3029568956C547475151 @default.
• W3029568956 hasConceptScore W3029568956C55493867 @default.
• W3029568956 hasConceptScore W3029568956C66999527 @default.
• W3029568956 hasConceptScore W3029568956C86803240 @default.
• W3029568956 hasConceptScore W3029568956C89423630 @default.
• W3029568956 hasConceptScore W3029568956C98274493 @default.
• W3029568956 hasIssue "9" @default.
• W3029568956 hasLocation W30295689561 @default.
• W3029568956 hasOpenAccess W3029568956 @default.
• W3029568956 hasPrimaryLocation W30295689561 @default.
• W3029568956 hasRelatedWork W1986158801 @default.
• W3029568956 hasRelatedWork W2037592724 @default.
• W3029568956 hasRelatedWork W2077687141 @default.
• W3029568956 hasRelatedWork W2089548909 @default.
• W3029568956 hasRelatedWork W2128367912 @default.
• W3029568956 hasRelatedWork W2132279045 @default.
• W3029568956 hasRelatedWork W2185322928 @default.
• W3029568956 hasRelatedWork W2346878574 @default.
• W3029568956 hasRelatedWork W2347160355 @default.
• W3029568956 hasRelatedWork W2358294349 @default.
• W3029568956 hasRelatedWork W2366209661 @default.
• W3029568956 hasRelatedWork W2367120779 @default.
• W3029568956 hasRelatedWork W2378049470 @default.
• W3029568956 hasRelatedWork W2418660689 @default.
• W3029568956 hasRelatedWork W2419547384 @default.
• W3029568956 hasRelatedWork W2467783085 @default.
• W3029568956 hasRelatedWork W2538798134 @default.
• W3029568956 hasRelatedWork W2965746232 @default.
• W3029568956 hasRelatedWork W3031982530 @default.
• W3029568956 hasRelatedWork W3149713004 @default.
• W3029568956 hasVolume "50" @default.
• W3029568956 isParatext "false" @default.
• W3029568956 isRetracted "false" @default.
• W3029568956 magId "3029568956" @default.
• W3029568956 workType "article" @default.