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- W3029647435 abstract "Objective To investigate the alleviation of liver ischemia/reperfusion injury (IRI) by inhibiting the Caspase-8 expression. Methods The eukaryotic expression vector of small hairpin RNA targeting rat Caspase-8 gene was constructed. The Lewis rats were randomly divided into 3 groups (n = 8 each):(1) sham group: the rats with laparotomy did not undergo the liver IRI; (2) PBS group:PBS was injected through portal vein 48 h before operation; (3) shRNA group: Caspase-8 shRNA was injected through portal vein 48 h before operation. The left lateral and median lobes of the liver were rendered ischemia for 40 min resulting in a segmental (70%) hepatic ischemia. The serum ALT and AST levels were detected 6 h, 12 h, 24 h, 3 days, 5 days and 7 days after reperfusion respectively.The mRNA expression level of Caspase-8, apoptosis index, concentrations of the malondialdehyde (MDA) and superoxide dismutase (SOD) in the liver tissue were detected. Results Compared with PBS group, the ALT and AST levels were significantly decreased in shRNA group 6 h, 12 h and 24 h after reperfusion (P<0.05), Caspase-8 mRNA expression level, apoptosis index (22.33%±4.28% vs 35.24%±2.33%, P<0.05) and concentrations of MDA in the liver tissue (94.5±11.2 vs 133.5±12.4 nmol/mg, P<0.05) were significantly decreased, and the SOD activity was significantly increased (21.6±3.7 vs 12.2±3.1 U/nag, P < 0.05). Conclusion Inhibiti0on of the Caspase-8 expression by RNA interference can protect the liver IRI.Key words: Liver; Reperfusion iniury; Caspase 8; RNA interference" @default.
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- W3029647435 date "2009-11-20" @default.
- W3029647435 modified "2023-10-15" @default.
- W3029647435 title "Liver ischemia/reperfusion injury in rats was relieved by inhibiting the Caspase-8 expression" @default.
- W3029647435 doi "https://doi.org/10.3760/cma.j.issn.0254-1785.2009.11.010" @default.
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