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- W3029671064 abstract "Objective To isolate,culture and identify the endometrial stem cells.Methods Human endometrial cells were isolated and dissociated enzymatically and mechanically from human endometrium.Stromal and epithelial cells were separated by two series of filters.Limiting dilution assay was performed to culture stromal cells and epithelial cells,and cell morphology and growth characteristics were observed.The expressions of vimentin and cytokeratin in stromal and epithelial cells were detected by immunofluorescent staining.After primary culture for 15 days,the colony formation rates were calculated,and the expressions of CD133,CD34,CD45,CD90,CD73 and CD29 in stromal and epithelial cells were detected by flow cytometry.Results The stromal cells were fibroblast-like and radially arranged,with positive expression of vimentin by immunofluorescent staining.The colony formation rate of stromal cells reached (1.34±0.44)％ after primary culture for 15 days.The epithelial cells were polygonal or oval,with positive expression of cytokeratin by immunofluorescent staining.None colony formation was found in epithelial cells after primary culture for 15 days.Flow cytometry showed positive expressions of CD29,CD90 and CD73 but negative expressions of CD34,CD45 and CD133 in the colony-forming stromal cells.Conclusion There are a few endometrial stem cells with colony formation ability and high proliferative potential in human body,which possibly originate from bone marrow mesenchymal stem cells.Key words: Adult stem cells; Endometrium; Immunophenotyping; Flow cytometry" @default.
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- W3029671064 date "2013-02-15" @default.
- W3029671064 modified "2023-09-23" @default.
- W3029671064 title "Isolation, culture and identification of human endometrial stem cells" @default.
- W3029671064 doi "https://doi.org/10.3760/cma.j.issn.1674-1927.2013.01.010" @default.
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