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- W3029684805 abstract "目的 建立并稳定HLA-B位点的参照链介导的构象分析(RSCA)系统,并鉴定RSCA分型策略的优势.方法提取27例造血干细胞移植患者及63名供者的外周血DNA,采用RSCA进行HLA-B位点分型,并与序列特异性引物聚合酶链反应技术(PCR-SSP)分型结果进行比较,对于疑难标本采用测序鉴定.结果在90份标本中,87份(96.7%)可明确判定结果,其中有1份标本与PCR-SSP结果不一致,经测序鉴定RSCA结果正确;67份(74.4%)可直接指定等位基因型别.此外,1份标本(1.1%)因PCR扩增结果太弱而未能获得RSCA分型结果,2份标本只能确定1条等位基因,经测序发现另1条等位基因为已知等位基因,但RSCA数据库中没有其数据.随机选择20份标本进行RSCA重复性实验,重复率为100%.在PCR-SSP方法分型中,约有10%的标本需要重复1～3次方能判定结果.结论RSCA具有灵敏、特异、分辨率高、重复性好、可发现变异基因及新的等位基因、大规模和低成本等优点,适用于大规模的无关移植供、受者的选择。" @default.
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- W3029684805 date "2005-05-20" @default.
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- W3029684805 title "HLA-B locus genotyping with reference strand mediated conformation analysis (RSCA) system in clinical usage" @default.
- W3029684805 doi "https://doi.org/10.3760/cma.j.issn.0254-1785.2005.05.013" @default.
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