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- W3029693385 abstract "Objective To study the effect of 3 different organ storage solutions commonly used on donor hepatocytic apoptosis.Methods The donor livers were separately flushed and preserved for 24 hwith UW,HC-A and WMO-1 solutions.The orthotopic liver transplantation in(OLT)rats were performed and the survival rate within 3-days were observed.The apoptosis of donor liver in different time points of 3 storage solutions was detected by in situ terminal labeling technique(TUNEL method).Results The apoptosis occurred 12 h after the liver preservation with 3 storage solutions.The apoptotic index(AI)in HC-A and WMO-1 groups WaS higher than Uw group(P＜0.001).In UW preserved group,the AI was increased rapidly following warm oxygenated reperfusion of liver graft preserved in UW solution for 24 h after transplantation.3-days survival rate of OLT rats in UW group was 60%.significantly higher than in HGA and WMO-1 groups(P＜0.01).Conclusions There might be some difference in the constitution between the UW solution and HC-A or WMO-1 solutions.The critical factor that caused the apoptosis of transplanted liver cell might be the oxygenated reperfusion following the cold ischemia,and that was the main reason for primary graft nonfunction following liver transplantation.But the injury to hepatocytes caused by apoptosis and difference of organ storage solutions was also one of the important factors that couldn't be neglected.Key words: Organ preservation; Liver; Cell apoptosis" @default.
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- W3029693385 date "2000-01-20" @default.
- W3029693385 modified "2023-09-25" @default.
- W3029693385 title "The effect of different organ storage solutions on apoptosis in rat liver preservation" @default.
- W3029693385 doi "https://doi.org/10.3760/cma.j.issn.0254-1785.2000.01.016" @default.
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