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• W3029737549 endingPage "107645" @default.
• W3029737549 startingPage "107645" @default.
• W3029737549 abstract "Lactococcus lactis, a homofermentative lactic acid bacterium, is widely applied in fermentation industry. In this study, a reductive TCA pathway was constructed in L. lactis for the biosynthesis of malic acid, a C4-dicarboxylic acid platform molecule. First, genes encoding malate dehydrogenase (mdh) from Actinobacillus succinogenes and pyruvate carboxylase (pycAY715T) from L. lactis were expressed in L. lactis NZ9000 to establish the reductive TCA pathway. The gene encoding lactate dehydrogenase (ldh) was simultaneously deleted. Malic acid was accumulated in the resulting strain NZ02-1 with a titer of 1.92 g/L. Subsequently, genes including ldhB, ldhX, als, and pfl, were deleted, increasing the titer to 3.13 g/L in strain NZ03. A promoter library was developed based on the core region of the phosphofructose kinase promoter Ppfk, and a stronger, constitutive promoter was screened for the enhanced expression of pycAY715T, leading to an increased titer of 5.75 g/L in strain NZ20. Through fed-batch fermentation, the titer in strain NZ20 was boosted to 14.1 g/L with a yield of 0.19 g/g glucose. Through a dual-phase fermentation, the titer was improved to 20.52 g/L with a yield of 0.29 g/g. Our study enabled the biosynthesis of malic acid in L. lactis, expanding the application of this strain as a chassis strain in fermentation industry." @default.
• W3029737549 created "2020-06-05" @default.
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• W3029737549 date "2020-09-01" @default.
• W3029737549 modified "2023-09-25" @default.
• W3029737549 title "Enabling the biosynthesis of malic acid in Lactococcus lactis by establishing the reductive TCA pathway and promoter engineering" @default.
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• W3029737549 doi "https://doi.org/10.1016/j.bej.2020.107645" @default.
• W3029737549 hasPublicationYear "2020" @default.
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