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- W3029749523 abstract "Objective To construct human umbilical cord-derived mesenchymal stem cells which were modified by glial cell line-derived neurotrophic factor gene. Methods The target gene in was identi- fied. PA317 cells were transfected with recombinant retroviral vector pLXSN-glial cell line-derived neuro- trophic factor （GDNF） using liposomes. The retrovirus titers were determined. Then the umbilical cord-de- rived mesenchymal stem cells （UCMSCs） were infected by pLXSN-GDNF. Finally, the immunocytochem- istry and reverse transcription-polymerase chain reaction （RT-PCR） were used to detect the expression of GDNF. Results The target gene was correct. The pLXSN-GDNF vector was successfully transfected into the PA317 cells using liposomes. The highest virus titre of the clone was 1 x 104 CFU/ml. Cells in both groups were immunohistochemically positive for GDNF expression. Staining for GDNF was more prominent in the UCMSCs infected with pLXSN-GDNF. RT-PCR revealed the UCMSCs modified by GDNF gene ex- pressed GDNF mRNA obviously higher than the UCMSCs which were not decorated by GDNF gene （P 〈 0. 01 ）. Conclusion The UCMSCs modified by GDNF were successfully constructed.Key words: Glial cell line-derived neurotrophic factor; Umbilical cord-derived mesenchymal stem cells; Retrovirus vector" @default.
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- W3029749523 date "2012-12-08" @default.
- W3029749523 modified "2023-09-23" @default.
- W3029749523 title "Transfection of recombinant retrovirus vector pLXSN-glial cell line-derived neurotrophic factor into umbilical cord-derived mesenchymal stem cells" @default.
- W3029749523 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2012.12.073" @default.
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