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- W3029777741 abstract "ObjectiveRecombinant human G-CSF(Rh-GCSF) is both an initiator of hematopoietic stem cells and an inducer of immune tolerance, and can reduce graft versus host disease (GVHD). Interferon regulatory factor 8 (IRF8) antagonizes the effect of ROR γ t and inhibites the differentiation of mouse naive T cells into Th17 cells. The aim of this study is to investigate the effects of rhG-CSF mobilization on the gene expression and protein level of IRF8, the proportions of Th17 and Treg cells in peripheral blood, and the role of negative regulatory factor IL-27 of Th17 differentiation.MethodsWe collected peripheral blood from 10 hematopoietic stem cell transplantation donors before and after the mobilization of rhG-CSF (fifth day). CD4+ T cells were isolated by immunomagnetic beads. IRF8 mRNA and protein in CD4+ T cells were detected by qRT-PCR and western blotting, respectively. The percentages of Th17 (CD3+ CD8-IL-17) and Treg cell (CD4+ CD25+ Foxp3+) were measured by flow cytometry. The concentrations of IL-17 and IL-27 in serum were detected by ELISA.ResultsThe percentages of mononuclear cells in peripheral blood before and after mobilization were as below: Th17 cells (2.597±1.993)% vs. (0.355±0.129)% (P<0.01); Treg cells (0.468±0.522)% vs. (1.491±1.216)% (P<0.01). In addition, the cytokine concentrations in serum before and after mobilization were as below: IL-17A (21.75±18.77) pg/ml vs. (15.53±10.89) pg/ml (P<0.01); IL-27 (5 180.06±1 860.75) pg/ml vs. (3 806.56±1 658.89) pg/ml (P<0.01). The expression of IRF8 mRNA increased by 1.69 folds, and the expression of IRF8 protein was significantly higher after than before the mobilization.ConclusionsRhG-CSF inhibits Th17 cells, and promotes the differentiation of Treg cells in donor peripheral blood, and induces immune tolerance. It may be related to RhG-CSF promoting IRF8 expression in CD4+ T cells. IL-27 and IRF8 negatively regulate Th17 cell differentiation.Key words: Recombinant human granulocyte colony stimulating factor; Th17 cells; Regulatory T cells; Interferon regulatory factor 8; Interleukin-27" @default.
- W3029777741 created "2020-06-05" @default.
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- W3029777741 date "2019-06-01" @default.
- W3029777741 modified "2023-09-26" @default.
- W3029777741 title "Mechanism of recombinant human G-CSF regulating the expressions of IL-27 and IRF8 in inducing donor-recipient immune tolerance" @default.
- W3029777741 doi "https://doi.org/10.3760/cma.j.issn.1007-1245.2019.11.028" @default.
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